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Los picos de calcio en los pelos de las raíces de las plantas que responden a las señales de nodulación de Rhizobium
D W Ehrhardt1, R Wais, S R Long
1Howard Hughes Medical Institute, Department of Biological Sciences, Stanford University, California 94305-5020, USA.
Cell
|May 31, 1996
Resumen
Las moléculas de señal de lipochitooligosacárido de Rhizobium (LCO) desencadenan picos de calcio en los pelos de las raíces de las leguminosas, un paso clave en el desarrollo de nódulos. Un mutante no nodulante que carece de estos picos de calcio sugiere un defecto en la percepción temprana de la señal.
Área de la Ciencia:
- Interacciones entre plantas y microbios.
- La señalización molecular en las plantas.
- La simbiosis de las leguminosas.
Sus antecedentes:
- La simbiosis de las legumbres involucra a las bacterias Rhizobium que forman nódulos de raíces fijadoras de nitrógeno.
- Las moléculas de señal de lipochitooligosacárido (LCO) de Rhizobium inician esta simbiosis.
- La vía precisa de transducción de señal basada en plantas para los LCO sigue siendo en gran medida desconocida.
Objetivo del estudio:
- Para investigar el papel del calcio citoplasmático en las respuestas de las plantas a las moléculas de señal LCO.
- Para determinar si el aumento de calcio inducido por LCO es esencial para la nodulación.
- Para examinar la señalización del calcio en un mutante de alfalfa no nodulante.
Principales métodos:
- Utilizó tintes reporteros sensibles al calcio inyectados en los pelos de la raíz del huésped.
- Se empleó el análisis de imágenes para detectar y cuantificar las fluctuaciones del calcio en el citoplasma.
- Evaluó la respuesta de aumento de calcio a las LCO en alfalfa de tipo silvestre y mutante.
Principales resultados:
- La aplicación de LCO indujo picos localizados y periódicos en el calcio citoplasmático dentro de los pelos de la raíz después de un retraso.
- Los requisitos estructurales para que los LCOs provocaran respuestas de nodulación fueron idénticos a los del calcium spiking.
- Un mutante de alfalfa no nodulante exhibió una ausencia completa de picos de calcio inducidos por LCO.
Conclusiones:
- El aumento de calcio en el citoplasma es una respuesta rápida y temprana de los pelos de las raíces de las legumbres a las señales de Rhizobium LCO.
- El aumento de calcio es un componente necesario del proceso de nodulación, probablemente involucrado en la percepción de la señal.
- El defecto del mutante no nodulante en el aumento del calcio indica un bloqueo en la percepción o transducción temprana de la señal LCO.
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