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Civ1 (CAK in vivo), una nueva quinasa activadora de Cdk, también conocida como Cdk

J Y Thuret¹, J G Valay, G Faye

¹Service de Biochimie et Génétique Molécularie, Gif-sur-Yvette, France.

|August 23, 1996

Resumen

Los investigadores descubrieron Civ1, una nueva quinasa activadora de Cdk (CAK) en la levadura. Civ1 es esencial para la viabilidad celular, fosforilando Cdc28 para regular la división celular y la expresión génica.

Área de la Ciencia:

Biología Molecular Biología Molecular
Biología celular Biología celular.
La bioquímica es la bioquímica.

Sus antecedentes:

Las cinasas proteicas dependientes de ciclina (Cdks) regulan la división celular y la expresión génica.
La activación de Cdk normalmente requiere la unión de ciclina y la fosforilación por una cinasa activadora de Cdk (CAK).

Objetivo del estudio:

Identificar y caracterizar una nueva actividad de CAK en Saccharomyces cerevisiae (S. cerevisiae).
Para investigar la relevancia fisiológica de este nuevo CAK in vivo.

Principales métodos:

Análisis de similitud de secuencia para identificar posibles candidatos a CAK.
Análisis bioquímicos para confirmar la actividad de la quinasa y la especificidad del sustrato.
Análisis genético utilizando mutantes sensibles a la temperatura para evaluar la función in vivo.

Principales resultados:

Identificación de Civ1 (CAK in vivo), un nuevo CAK con similitud de secuencia con Cdks pero activo como monómero.
Civ1 se une directamente y fosforila Cdc28, lo que permite la posterior unión y activación de la ciclina.
El gen CIV1 es esencial para la viabilidad de la levadura, y un mutante civ1-4 muestra defectos condicionales en la fosforilación y actividad de Cdc28.

Conclusiones:

Civ1 es el primer CAK identificado con evidencia genética de relevancia fisiológica esencial in vivo.
Civ1 puede representar un miembro fundador de una nueva familia de kinasas.
Civ1 juega un papel crítico en la regulación de la división celular y la expresión génica en S. cerevisiae.

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