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Los factores asociados a TBP generalmente no son necesarios para la activación transcripcional en levaduras
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Nature
|September 12, 1996
Resumen
Los factores asociados con TBP (TAF) son esenciales para el crecimiento de la levadura, pero generalmente no se requieren para activar la transcripción. El agotamiento de TAF afecta a algunos promotores sin TATA, revelando roles específicos en la regulación génica.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Genética La genética.
- La bioquímica es la bioquímica.
Sus antecedentes:
- TFIID es un factor de transcripción clave para la transcripción de la ARN polimerasa II (Pol II) en eucariotas.
- Consiste en la proteína de unión a TATA (TBP) y los factores asociados a la TBP (TAF).
- Si bien el papel de TBP está bien definido, las funciones de TAF, especialmente in vivo, siguen siendo menos comprendidas.
Objetivo del estudio:
- Para investigar las funciones transcripcionales específicas in vivo de los TAF en la levadura.
- Determinar la necesidad de TAF para la transcripción basal y la activación por factores específicos.
Principales métodos:
- Utilizó un nuevo método de doble cierre para crear alelos condicionales para el agotamiento de TAF en la levadura.
- Evaluó el impacto del agotamiento de TAF en la transcripción de varios promotores, incluidos los sin TATA.
- Se evaluó el efecto del agotamiento de TAF en la mejora de la transcripción por activadores conocidos.
Principales resultados:
- El agotamiento de TAF redujo la transcripción de ciertos promotores que carecían de elementos TATA.
- Sorprendentemente, el agotamiento de TAF tuvo un impacto mínimo en la mejora transcripcional por varios activadores.
- Esto sugiere que los TAF no son universalmente requeridos para la activación transcripcional en levaduras.
Conclusiones:
- Los TAF juegan un papel en la regulación de la transcripción de promotores específicos sin TATA.
- El requisito general de TAF para la transcripción mediada por activadores en levaduras es menos significativo de lo que se suponía anteriormente.
- Se necesita más investigación para dilucidar los roles precisos de los TAF individuales en la transcripción de la levadura.
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