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La cooperación de Stat2 y p300/CBP en la señalización inducida por el interferón alfa
S Bhattacharya1, R Eckner, S Grossman
1Dana-Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts 02115, USA.
Nature
|September 26, 1996
Resumen
La proteína E1A del adenovirus inhibe la respuesta antiviral del interferón alfa (IFN-alfa) al bloquear la interacción entre Stat2 y p300/CBP. Esta interrupción impide la activación de los genes de defensa celular, lo que ayuda a la replicación viral.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Virología Virología.
- Inmunología Inmunología.
Sus antecedentes:
- La señalización de interferón-alfa (IFN-alfa) activa los genes celulares de defensa antiviral a través del factor de transcripción ISGF3.
- Se sabe que la proteína E1A del adenovirus interfiere con la respuesta IFN-alfa, promoviendo la replicación viral.
- ISGF3 está compuesto por las proteínas Stat1, Stat2 y p48.
Objetivo del estudio:
- Para dilucidar el mecanismo por el cual el adenovirus E1A inhibe la respuesta IFN-alfa.
- Para investigar la interacción entre los componentes de ISGF3 y los adaptadores de transcripción celular dirigidos por E1A.
- Para determinar la importancia funcional de estas interacciones para la replicación viral.
Principales métodos:
- Los ensayos de coinmunoprecipitación detectan las interacciones proteína-proteína.
- Análisis de los dominios de proteínas involucrados en la unión.
- Pruebas de transactivación para medir la expresión génica.
- Estudios de inhibición para evaluar el efecto de E1A en la señalización de IFN-alfa.
Principales resultados:
- p300 y la proteína de unión al CREB (CBP) interactúan específicamente con Stat2, un componente del ISGF3.
- Esta interacción ocurre entre la región rica en cisteína e histidina de p300/CBP y el dominio carboxiterminal de Stat2.
- El dominio carboxiterminal de Stat2 posee un potencial de transactivación, el cual es dependiente de la unión p300/CBP.
- El adenovirus E1A reprime la transactivación de Stat2 y la transcripción inducida por IFN-alfa al inhibir la función p300/CBP.
Conclusiones:
- El adenovirus E1A inhibe la respuesta antiviral activada por IFN-alfa al interrumpir la interacción entre Stat2 y p300/CBP.
- Este mecanismo proporciona una nueva forma en que E1A suprime las defensas celulares.
- La unión de E1A a p300/CBP es funcionalmente significativa para la replicación del adenovirus.
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