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Un socio transcripcional para las proteínas MAD en la señalización de TGF-beta
X Chen1, M J Rubock, M Whitman
1Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Nature
|October 24, 1996
Resumen
La señalización del factor de crecimiento transformador-beta (TGF-beta) es crucial para el desarrollo del mesodermo en los embriones de Xenopus. Un nuevo factor de transcripción de hélice alada y XMAD2 forman un complejo para regular la expresión génica de Mix.2.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Biología Molecular Biología Molecular
- Genética La genética.
Sus antecedentes:
- La superfamilia del factor de crecimiento transformador-beta (TGF-beta) juega un papel crítico en la inducción temprana del mesodermo embrionario.
- La activina, un miembro similar a la activina de la superfamilia TGF-beta, desencadena respuestas genéticas específicas en Xenopus.
- El gen Mix.2 es un gen de respuesta inmediata y temprana activado por una señalización similar a la activina.
Objetivo del estudio:
- Para identificar la actividad de unión al ADN que regula la expresión génica de Mix.2.
- Para caracterizar los componentes moleculares involucrados en la señalización de la superfamilia TGF-beta en la embriogénesis de Xenopus.
- Para dilucidar el mecanismo por el cual las señales de TGF-beta regulan la expresión génica en embriones tempranos.
Principales métodos:
- Clonado de un nuevo factor de transcripción de hélice alada responsable de la unión con el elemento ARE del promotor Mix.2.
- Investigando la interacción entre el factor de transcripción identificado y el transductor de señal TGF-beta XMAD2.2.
- Desarrollo de un modelo para la transducción de señales de la superfamilia TGF-beta en la regulación genética embrionaria temprana.
Principales resultados:
- Se identificó un nuevo factor de transcripción de hélice alada como el componente de unión al ADN específico del sitio para el ARE. del promotor de Mix.2.
- Este factor de transcripción es un objetivo directo de la señalización de la superfamilia TGF-beta.
- XMAD2 forma un complejo con este factor de transcripción de una manera dependiente de la activina, creando un complejo de unión de ARE activado.
Conclusiones:
- Las señales de la superfamilia TGF-beta regulan la expresión de genes específicos, como Mix.2, durante la embriogénesis temprana de Xenopus.
- Un nuevo factor de transcripción y XMAD2 son componentes clave en esta vía de señalización.
- Un modelo propuesto explica el mecanismo de la regulación génica mediada por TGF-beta en el desarrollo embrionario.
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