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El gen supresor de tumores parcheado codifica un receptor candidato para Sonic hedgehog
D M Stone1, M Hynes, M Armanini
1Department of Neuroscience, Genentech, Inc., South San Francisco, California 94080, USA.
Nature
|November 14, 1996
Resumen
La proteína Sonic hedgehog (Shh) se une a Patched (Ptc), un supresor de tumores. Patched interactúa con Smoothened (Smo), lo que sugiere que Ptc actúa como un receptor Shh en el desarrollo de los vertebrados.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Biología Molecular Biología Molecular
- Investigación del cáncer Investigación del cáncer.
Sus antecedentes:
- Sonic hedgehog (Shh) es una proteína crucial que regula el desarrollo, el patrón y el crecimiento de los vertebrados.
- Patched (Ptc) es una conocida proteína supresora de tumores implicada en el carcinoma basocelular.
- Smoothened (Smo) es una proteína clave en las vías de señalización del desarrollo.
Objetivo del estudio:
- Para investigar la interacción entre Sonic hedgehog (Shh) y Patched (Ptc).
- Para explorar la relación entre parcheado (Ptc) y suavizado (vSmo) en vertebrados.
- Para dilucidar el mecanismo molecular de la señalización Shh en el desarrollo.
Principales métodos:
- Pruebas de unión a proteínas para demostrar una interacción de alta afinidad entre Shh y Ptc.
- Co-inmunoprecipitación para mostrar la formación de complejos físicos entre Ptc y vSmo.
- Análisis de expresión para determinar la co-localización de vPtc y vSmo en varios tejidos.
Principales resultados:
- La proteína Sonic hedgehog (Shh) se une con una alta afinidad a Patched (Ptc).
- El Ptc de los vertebrados forma un complejo físico con un homólogo suavizado de vertebrados (vSmo).
- vSmo se coexpresa con vPtc pero no se une directamente con Shh.
Conclusiones:
- Parcheado (Ptc) funciona como un receptor para Sonic hedgehog (Shh).
- Vertebrate Smoothened (vSmo) es un componente de señalización asociado con el receptor Ptc.
- Estos hallazgos apoyan un modelo para la transducción de la señal Shh que involucra Ptc y vSmo.
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