Video Experimental Relacionado
Updated: Jul 11, 2026
10:16
Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
Published on: June 28, 2018
Los coactivadores transcripcionales p300 y CBP son las histonas acetiltransferasas
V V Ogryzko1, R L Schiltz, V Russanova
1Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-2753, USA.
Cell
|November 29, 1996
Resumen
La proteína p300/CBP actúa como una nueva histona acetiltransferasa, modificando las histonas del núcleo en los nucleosomas. Este hallazgo revela una nueva clase de acetiltransferasas involucradas en la regulación génica.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- La epigenética es la epigenética.
- Regulación genética Reglamento genético.
Sus antecedentes:
- p300/CBP funciona como un adaptador de transcripción, integrando las señales de los activadores.
- Los factores celulares y virales se dirigen a p300/CBP para influir en la transcripción y el ciclo celular.
- El PCAF, un factor asociado p300/CBP, tiene actividad intrínseca de la histona acetiltransferasa.
Objetivo del estudio:
- Para investigar la actividad enzimática de p300/CBP.
- Para determinar si p300/CBP posee actividad de la histona acetiltransferasa (HAT).
- Caracterizar la nueva clase de acetiltransferasas representadas por p300/CBP.
Principales métodos:
- Análisis bioquímicos para evaluar las interacciones de las proteínas.
- Los ensayos de actividad enzimática miden la acetilación de la histona.
- Análisis de los motivos conservados en las acetiltransferasas.
Principales resultados:
- p300/CBP demuestra actividad de la histona acetiltransferasa.
- p300/CBP acetila las cuatro histonas centrales dentro de los nucleosomas.
- p300/CBP carece del motivo conservado típico de otras acetiltransferasas, lo que indica una nueva clase.
Conclusiones:
- p300/CBP es una nueva histona acetiltransferasa, distinta de las enzimas caracterizadas anteriormente.
- p300/CBP probablemente acetila los nucleosomas junto con el PCAF.
- Estos hallazgos amplían la comprensión de la regulación epigenética y el control transcripcional.
Videos de Conceptos Relacionados
02:36RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
02:04Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
02:42Eukaryotic Transcription Activators
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
02:04Co-activators and Co-repressors
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...