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Arquitectura de microtúbulos especificada por una isoforma de beta-tubulina
E C Raff1, J D Fackenthal, J A Hutchens
1Department of Biology and Indiana Molecular Biology Institute, Indiana University, Bloomington, IN 47405, USA. eraff@bio.indiana.edu
Science (New York, N.Y.)
|January 3, 1997
Resumen
Una proteína beta-tubulina de polilla, cuando se expresa en las moscas de la fruta, alteró la estructura de los microtúbulos de la mosca. Esto demuestra que las proteínas beta-tubulinas de diferentes especies pueden dirigir la arquitectura de los microtúbulos.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología del desarrollo Biología del desarrollo.
- Genética La genética.
Sus antecedentes:
- La espermatogénesis en Drosophila melanogaster se basa en una beta-tubulina específica (beta2).
- Existe un motivo de secuencia conservada en los terminales carboxílicos de las beta-tubulinas axonémicas en varias especies.
- La equivalencia funcional de las beta-tubulinas ortólogas de diferentes especies sigue siendo en gran medida inexplorada.
Objetivo del estudio:
- Para investigar si las proteínas de beta-tubulina ortólogas de diferentes especies exhiben equivalencia funcional.
- Para determinar si un homólogo de la polilla beta-tubulina puede funcionar en el contexto de la espermatogénesis de Drosophila.
Principales métodos:
- La expresión del homólogo de la beta2-tubulina de Heliothis virescens en los testículos de Drosophila melanogaster.
- Coexpresión de la beta-tubulina de la polilla introducida con la endógena Drosophila beta2-tubulina.
- Análisis microscópico para evaluar la estructura de microtúbulos y el número de protofilamentos.
Principales resultados:
- La isoforma beta-tubulina de polilla introducida se coexpresó con éxito con la beta2-tubulina endógena de Drosophila.
- La polilla beta-tubulina impuso su característica estructura de 16 protofilamentos a un subconjunto de microtúbulos de Drosophila.
- Normalmente los microtúbulos de 13 protofilamentos en Drosophila fueron alterados en su arquitectura por la presencia de la polilla ortholog.
Conclusiones:
- Las proteínas de beta-tubulina ortólogas de diferentes especies no siempre son funcionalmente equivalentes en términos de dictar la arquitectura de los microtúbulos.
- El componente beta-tubulina puede dirigir activamente el número de protofilamentos y la organización estructural de los microtúbulos.
- Este hallazgo tiene implicaciones para la comprensión de la evolución de los microtúbulos y la especificidad de las estructuras citoesqueléticas.
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