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La supresión de la apoptosis inducida por c-Myc por la señalización Ras a través de PI(3) K y PKB
A Kauffmann-Zeh1, P Rodriguez-Viciana, E Ulrich
1Imperial Cancer Research Fund, London, UK.
Nature
|February 6, 1997
Resumen
La señalización Ras activa vías contradictorias, suprimiendo la apoptosis inducida por c-Myc a través de la fosfatidilinositol-3-cinasa (PI(3) K) y la proteína quinasa B/Akt, pero promoviendo la apoptosis a través de la vía Raf.
Área de la Ciencia:
- Biología celular Biología celular.
- La oncología molecular es una oncología molecular.
- Transducción de señales de transducción de señales.
Sus antecedentes:
- La viabilidad celular se basa en factores de supervivencia que inhiben la apoptosis, un proceso de muerte celular programada.
- Los defectos en las vías antiapoptóticas están vinculados a patologías como el cáncer, donde las células tumorales deben evadir la apoptosis.
- La fosfatidilinositol-3-cinasa (PI(3) K) es crucial para la transducción de la señal intracelular y la señalización de supervivencia.
Objetivo del estudio:
- Investigar las funciones de PI(3)K, Ras, PKB/Akt y p70S6K en la modulación de la apoptosis inducida por la oncoproteína c-Myc.
- Aclarar el doble papel de Ras en la regulación de la viabilidad celular a través de distintas vías de señalización.
Principales métodos:
- Inducción de la apoptosis de los fibroblastos por c-Myc.
- Análisis de las vías de señalización Ras, PI(3)K, PKB/Akt y p70S6K.
- Evaluación de la viabilidad celular y la modulación de la apoptosis.
Principales resultados:
- La activación Ras de PI(3) K suprime la apoptosis inducida por c-Myc mediante la activación de PKB/Akt, pero no de p70S6K.
- Ras también promueve la apoptosis a través de la vía Raf, lo que indica una señalización contradictoria.
- La activación Ras de PI(3)K y PKB/Akt es esencial para suprimir la apoptosis inducida por c-Myc.
Conclusiones:
- Ras exhibe un doble papel en la viabilidad celular, capaz de suprimir y promover la apoptosis.
- La vía PI(3)K/PKB/Akt es un mediador clave de la supresión dependiente de Ras de la apoptosis inducida por c-Myc.
- La apoptosis inducida por Ras puede limitar la proliferación de células somáticas con mutaciones Ras oncogénicas.
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