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Las desacetilasas de histona y SAP18, un nuevo polipéptido, son componentes de un complejo Sin3 humano
Y Zhang1, R Iratni, H Erdjument-Bromage
1Howard Hughes Medical Institute, Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854, USA.
Cell
|May 2, 1997
Resumen
El complejo mamífero Sin3 (mSin3), implicado en la expresión génica, interactúa con las histonas desacetilasas (HDAC1/HDAC2). Las nuevas proteínas SAP18 y SAP30 fueron identificadas como parte de este complejo, con SAP18 interactuando directamente con mSin3 para reprimir la transcripción.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Reglamento genético Reglamento genético.
- La epigenética es la epigenética.
Sus antecedentes:
- Las modificaciones de la histona son cruciales para la regulación de la expresión génica.
- El mamífero Sin3 (mSin3) es un represor de la transcripción involucrado en estos procesos.
Objetivo del estudio:
- Para investigar la composición del complejo que contiene mSin3.
- Para identificar nuevas proteínas asociadas con mSin3.
- Para aclarar el papel de estas proteínas asociadas en la represión transcripcional.
Principales métodos:
- Co-inmunoprecipitación para identificar los componentes complejos.
- Aislamiento y caracterización del ADNc.
- Pruebas de transcripción in vivo.
Principales resultados:
- El complejo mSin3 incluye las histonas desacetilasas HDAC1 y HDAC2.2.
- Se identificaron dos nuevos polipéptidos asociados a mSin3, SAP18 y SAP30.
- SAP18 interactúa directamente con mSin3 y media la represión transcripcional cuando está atado a un promotor.
Conclusiones:
- El complejo mSin3 es un conjunto de múltiples proteínas involucrado en la regulación epigenética de los genes.
- SAP18 es un nuevo componente del complejo mSin3 que contribuye a la represión transcripcional.
- Comprender el complejo mSin3 proporciona información sobre los mecanismos de silenciamiento de genes.
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