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Se requiere un gen similar a la acuaporina para la respuesta de autoincompatibilidad de la Brassica

S Ikeda¹, J B Nasrallah, R Dixit

¹Section of Plant Biology, Division of Biological Sciences, Cornell University, Ithaca, NY 14853, USA.

Science (New York, N.Y.)

|June 6, 1997

Resumen

La autoincompatibilidad en las flores de Brassica está regulada por un gen específico. Una mutación que afecta a este gen conduce a la autocompatibilidad, lo que sugiere que un canal de agua es crucial para el rechazo del polen.

Área de la Ciencia:

Biología reproductiva de las plantas Biología reproductiva de las plantas
Genética molecular genética molecular.
Fisiología celular La fisiología celular.

Sus antecedentes:

La autoincompatibilidad (SI) en Brassica impide la autopolinización, mediada por las quinasas del receptor del estigma.
La mutación recesiva "mod" interrumpe el SI, lo que lleva a la autocompatibilidad.
SI es vital para mantener la diversidad genética en las especies de Brassica.

Objetivo del estudio:

Para investigar la base molecular de la autocompatibilidad en las mutantes mod de Brassica.
Para identificar los genes involucrados en la vía SI.
Comprender el papel del transporte acuático en la polinización de la Brassica.

Principales métodos:

Análisis de la expresión génica en Brassica tipo salvaje y mutante mod.
Identificación de las transcripciones ausentes en los mutantes mod.
Correlación de la ausencia de genes con la pérdida de la autoincompatibilidad.

Principales resultados:

La mutación modificada está asociada con la ausencia de transcripciones de un gen relacionado con la acuaporina.
Es probable que este gen relacionado con la acuaporina esté involucrado en la respuesta SI.
La ausencia de las transcripciones de este gen se correlaciona con la ganancia de autocompatibilidad.

Conclusiones:

Un canal de agua, probablemente codificado por el gen identificado relacionado con la acuaporina, es esencial para la autoincompatibilidad de Brassica.
La regulación de la transferencia de agua al polen es un punto de control crítico en la polinización de Brassica.
La mutación mod proporciona información sobre los mecanismos moleculares que subyacen al aislamiento reproductivo de las plantas.

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