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Detection of Neu1 Sialidase Activity in Regulating TOLL-like Receptor Activation
Published on: September 7, 2010
La respuesta a la neurturina requiere un receptor ligado al GPI y la tirosina quinasa del receptor Ret
A Buj-Bello1, J Adu, L G Piñón
1School of Biological and Medical Sciences, University of St Andrews, Fife, UK.
Nature
|June 12, 1997
Resumen
La neurturina (NTN) y el GDNF son factores neurotróficos que promueven la supervivencia de las neuronas. Se señalan a través de complejos de receptores distintos pero similares que involucran a Ret y receptores específicos vinculados al GPI, lo que determina la especificidad del ligando.
Área de la Ciencia:
- La neurociencia es la neurociencia.
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
Sus antecedentes:
- El factor neurotrófico derivado de la línea de células gliales (GDNF) apoya la supervivencia neuronal y es crucial para el sistema nervioso y el desarrollo renal.
- El GDNF funciona a través de un complejo de receptores que incluye Ret y GDNFR-alpha.
- La neurturina (NTN) es un homólogo recientemente identificado del GDNF.
Objetivo del estudio:
- Clonar y caracterizar el receptor para la neurturina (NTN).
- Investigar los mecanismos de recepción de NTN y GDNF en la supervivencia neuronal.
Principales métodos:
- La clonación del receptor NTN, NTNR-alfa.
- Coexpresión de NTNR-alfa o GDNFR-alfa con Ret en las neuronas a través de microinyección.
- Evaluación de las respuestas de supervivencia neuronal a NTN y GDNF.
Principales resultados:
- NTNR-alfa, un homólogo de GDNFR-alfa, fue clonado y se encontró que está ampliamente expresado.
- La coexpresión de NTNR-alfa con Ret confiere una supervivencia específica de la NTN.
- La coexpresión de GDNFR-alfa con Ret confería una supervivencia específica del GDNF.
Conclusiones:
- GDNF y NTN utilizan receptores distintos vinculados a GPI (GDNFR-alfa y NTNR-alfa, respectivamente) que se asocian con Ret.
- La especificidad del ligando en la señalización GDNF/NTN está determinada por el componente del receptor ligado al GPI.
- Esto revela un mecanismo conservado para la señalización del factor neurotrófico a través de receptores multicomponentes.
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