Video Experimental Relacionado
Updated: Jun 25, 2026
08:56
Isolation of Murine Embryonic Hemogenic Endothelial Cells
Published on: June 17, 2016
Un requisito para Flk1 en la hematopoyesis primitiva y definitiva y la vasculogénesis
F Shalaby1, J Ho, W L Stanford
1Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
Cell
|June 13, 1997
Resumen
La tirosina quinasa del receptor Flk1 es esencial para el desarrollo de las células endoteliales y la formación de sangre en ratones. La falta de Flk1 impide que las células formen islas de sangre, lo que afecta tanto a la vasculogénesis como a la hematopoyesis.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- La hematopoyesis es la hematopoyesis.
- Biología de las células endoteliales.
Sus antecedentes:
- Las receptoras tirosina quinasas juegan un papel crítico en la señalización celular y el desarrollo.
- Flk1 (VEGFR2) es un receptor clave involucrado en la formación temprana de sangre y vasos sanguíneos.
Objetivo del estudio:
- Investigar el papel celular autónomo de Flk1 en las células madre embrionarias durante la vasculogénesis y la hematopoyesis.
- Determinar la contribución de las células deficientes de Flk1 a la formación de islas sanguíneas y al desarrollo hematopoyético.
Principales métodos:
- Generación de embriones quiméricos utilizando células madre embrionarias Flk1-/- .
- Análisis del desarrollo de células endoteliales y hematopoyéticas en sacos quiméricos de yema, hígados fetales y ratones adultos.
Principales resultados:
- Flk1 se requiere de forma celular autónoma para el desarrollo endotelial.
- Las células Flk1-/- no pudieron contribuir a la hematopoyesis primitiva y definitiva.
- La deficiencia de Flk1 deterioró la migración celular hacia la raya primitiva posterior y el saco vitelino, lo que dificulta la formación de islas sanguíneas.
Conclusiones:
- La señalización de Flk1 es indispensable para el desarrollo de las células endoteliales.
- Flk1 es crucial para la migración y la diferenciación de las células progenitoras hematopoyéticas.
- El estudio destaca el doble papel de Flk1 en los linajes endotelial y hematopoyético durante la embriogénesis temprana.
Videos de Conceptos Relacionados
01:10Mechanism of Angiogenesis
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
01:24Regulation of Angiogenesis and Blood Supply
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
01:21Hematopoiesis
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
01:20Overview of Hematopoiesis
Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
01:34Production of Formed Elements
Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...
Most HSCs commit to...
01:07Development of Blood Vessels
The development of the vascular system in a fetus is a complex and intricate process that begins as early as 15 to 16 days post-conception. This process starts outside the embryo, specifically in the mesoderm of the yolk sac, chorion, and connecting stalk. Approximately two days later, the formation of blood vessels occurs within the embryo itself.
The initial formation of this system is facilitated by the small amount of yolk present in the ovum and yolk sac. Blood vessels originate from...
The initial formation of this system is facilitated by the small amount of yolk present in the ovum and yolk sac. Blood vessels originate from...