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El coensamblaje de TRP y TRPL produce una conductividad operada por la tienda distinta
1Department of Biological Chemistry, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Cell
|June 27, 1997
Resumen
Las proteínas del potencial receptor transitorio (TRP) y del tipo TRP (TRPL) forman nuevos canales iónicos. La coexpresión revela un canal heteromultimerico que media las corrientes operadas por almacenamiento en los fotorreceptores de Drosophila.
Área de la Ciencia:
- Biología molecular y celular Biología molecular y celular.
- La neurociencia es la neurociencia.
- Fisiología del canal iónico Fisiología del canal iónico
Sus antecedentes:
- La proteína específica de la retina de Drosophila, el Potencial Receptor Transiente (TRP), es el miembro fundador de la familia de canales operados por almacenamiento conservado (SOC).
- Los estudios in vitro sugieren que TRP funciona como un SOC, mientras que la proteína relacionada, TRPL, es constitutivamente activa.
Objetivo del estudio:
- Investigar las consecuencias funcionales de la coexpresión de las proteínas TRP y TRPL.
- Determinar el mecanismo subyacente a la corriente activada por la luz en las células fotorreceptoras de Drosophila.
Principales métodos:
- Coexpresión de TRP y TRPL en un sistema de expresión adecuado.
- Registros electrofisiológicos para caracterizar las corrientes de los canales iónicos.
- Estudios de interacción de proteínas para evaluar la asociación TRP-TRPL.
Principales resultados:
- La coexpresión de TRP y TRPL genera una corriente única operada por almacenamiento, que se rectifica hacia afuera, distinta de las corrientes producidas por TRP o TRPL solo.
- Se confirmó la interacción directa entre las subunidades TRP y TRPL.
- La corriente observada está mediada por canales heteromultimeros formados por TRP y TRPL.
Conclusiones:
- Las proteínas TRP y TRPL interactúan directamente para formar canales heteromultimeros funcionales.
- Estos heteromultimers TRP-TRPL contribuyen a la corriente operada por almacenamiento en las células fotorreceptoras.
- La corriente activada por la luz en los fotorreceptores probablemente sea el resultado de una combinación de homomultimeros TRP y heteromultimeros TRP-TRPL.
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