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La recombinación de los dominios de proteínas facilitada por el plegamiento co-traducional en los eucariotas
1Cellular Biochemistry & Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
Nature
|July 24, 1997
Resumen
El plegamiento secuencial del dominio de la proteína en eucariotas ayuda a la evolución del genoma complejo. Esto contrasta con el plegamiento bacteriano, previniendo el plegamiento incorrecto y permitiendo nuevas estructuras proteicas.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología evolutiva Biología evolutiva.
- La genómica es la genómica.
Sus antecedentes:
- Los genomas complejos evolucionan a través de nuevas combinaciones de dominios de proteínas.
- El plegamiento de proteínas en polipéptidos modulares es esencial para este proceso.
Objetivo del estudio:
- Para investigar los mecanismos de plegado de los polipéptidos de dos dominios en los eucariotas frente a las bacterias.
- Comprender el papel del plegamiento en la evolución de las proteínas modulares.
Principales métodos:
- Análisis comparativo del plegamiento del polipéptido durante la traducción en modelos de eucariotas y Escherichia coli.
- Examen de las vías de plegado secuencial, co-traducional y post-traducional.
Principales resultados:
- La traducción eucariota facilita el plegamiento secuencial y co-traducional eficiente de los polipéptidos de dos dominios.
- Escherichia coli exhibe plegamiento posttraducional, lo que lleva a un plegamiento incorrecto intramolecular de los dominios de plegamiento simultáneos.
Conclusiones:
- El plegamiento de dominio secuencial en eucariotas puede ser crucial para la evolución de los polipéptidos modulares.
- Este proceso aumenta la probabilidad de que las fusiones genéticas produzcan estructuras proteicas funcionales.
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