Video Experimental Relacionado
Updated: May 12, 2026
12:19
Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks
Published on: November 10, 2016
Superenrolamiento positivo dependiente de ATP del ADN por la condensina 13S: una implicación bioquímica para la
Cell
|August 22, 1997
Resumen
La 13S condensina, un complejo proteico vital para la condensación cromosómica, muestra actividad de ATPasa estimulada por el ADN. Envuelve el ADN utilizando la hidrólisis de ATP, lo que potencialmente explica la compactación de la cromatina durante la mitosis.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
- La bioquímica es la bioquímica.
Sus antecedentes:
- La condensación del cromosoma mitótico es crucial para una división celular precisa.
- El complejo de condensina 13S, que contiene proteínas SMC, está implicado en este proceso.
- Comprender el mecanismo de la condensina es clave para la regulación del ciclo celular.
Objetivo del estudio:
- Para investigar las actividades bioquímicas de la condensina 13S.
- Para aclarar el papel de la hidrólisis de ATP en la función de la condensina.
- Para explorar la interacción de la condensina con la estructura del ADN.
Principales métodos:
- Análisis bioquímicos que miden la actividad de la ATPasa.
- Estudios de unión al ADN utilizando ADN estructurado.
- Ensayos de superenrolamiento con ADN circular cerrado y topoisomerasa I.
Principales resultados:
- La condensina 13S exhibe actividad de la ATPasa estimulada por el ADN.
- El complejo se une preferentemente a ADN estructurado como el ADN cruciforme.
- La condensina introduce superbobinas positivas en el ADN de una manera dependiente del ATP.
Conclusiones:
- La condensina 13S utiliza la hidrólisis de ATP para envolver el ADN.
- Este mecanismo de envoltura del ADN probablemente contribuye a la compactación de la cromatina durante la mitosis.
- Los hallazgos proporcionan información sobre la base molecular de la condensación cromosómica.
Videos de Conceptos Relacionados
02:21Chromatin Packaging
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter?
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
02:02DNA Topoisomerases
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. Type I...
Types and Mechanism of action
Topoisomerases are divided into two main types. Type I...
02:15Condensins
Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
02:11Forces Acting on Chromosomes
During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis.
Microtubules and motor proteins exert two types of forces on...
Microtubules and motor proteins exert two types of forces on...
02:15Condensins
Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...
01:32Chromatin Packaging
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...