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Strategies for Tracking Anastasis, A Cell Survival Phenomenon that Reverses Apoptosis
Published on: February 16, 2015
Fragmento de gelsolina generado por caspase-3: efector del cambio morfológico en la apoptosis
S Kothakota1, T Azuma, C Reinhard
1Chiron Corporation, Emeryville, CA 94608, USA.
Science (New York, N.Y.)
|October 10, 1997
Resumen
La caspasa-3 divide la gelsolina, una proteína clave en la apoptosis. Esta escisión causa cambios celulares como la fragmentación nuclear, destacando el papel de la gelsolina en la muerte celular programada.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
- La bioquímica es la bioquímica.
Sus antecedentes:
- La apoptosis es un proceso crítico en las células de mamíferos.
- La caspasa-3 es una proteasa clave que media la apoptosis.
- Los sustratos y efectores de la actividad de la caspasa-3 no se comprenden completamente.
Objetivo del estudio:
- Para identificar sustratos de la caspasa-3.
- Para investigar el papel de la hendidura de la gelsolina en la apoptosis.
Principales métodos:
- Cribado de los productos de traducción del ADNc para la sensibilidad a la caspasa-3.
- Ensayos de escisión in vivo e in vitro. análisis de escisión in vivo e in vitro.
- Morfología celular y análisis de fragmentación nuclear.
- Inducción de la apoptosis en los neutrófilos de tipo salvaje y con deficiencia de gelsolina.
Principales resultados:
- La gelsolina fue identificada como un sustrato directo de la caspasa-3.
- La escisión de la gelsolina mediada por Caspase-3 se produjo in vivo durante la apoptosis inducida por Fas.
- Los filamentos de actina cortados de gelsolina desgarrados independientemente del calcio.
- La expresión de la morfología apoptótica inducida por la gelsolina escindida (redondeamiento celular, desprendimiento, fragmentación nuclear).
- Los neutrófilos con deficiencia de gelsolina mostraron un retraso en el blab y la fragmentación del ADN durante la apoptosis.
Conclusiones:
- La gelsolina es un sustrato fisiológico de la caspasa-3.
- La gelsolina desgarrada contribuye a los cambios morfológicos característicos observados durante la apoptosis.
- Gelsolin juega un papel en la ejecución de la muerte celular programada.
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