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Componentes y dinámica de los complejos de replicación del ADN en S. cerevisiae: redistribución de las proteínas MCM
O M Aparicio1, D M Weinstein, S P Bell
1Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.
Cell
|October 23, 1997
Resumen
En la levadura, las proteínas clave como ORC, Cdc45p y MCM forman complejos de pre-replicación (pre-RC) en los orígenes de la replicación del ADN. Estas proteínas luego se mueven con horquillas de replicación durante la fase S, regulando la entrada de la replicación del ADN.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
- Genética La genética.
Sus antecedentes:
- La estructura de la cromatina en el origen de la replicación del ADN en S. cerevisiae cambia durante la fase G1.
- Esto sugiere que la asociación específica de la fase G1 de los factores de replicación con el ADN de origen regula la entrada en la fase S.
Objetivo del estudio:
- Para identificar los componentes de las proteínas de los complejos de pre-replicación (pre-RC).
- Caracterizar el comportamiento de los factores de replicación durante la transición G1/S y la fase S.
Principales métodos:
- Pruebas de inmunoprecipitación de la cromatina.
- Análisis de la asociación de proteínas con el ADN de origen y no origen durante el ciclo celular.
Principales resultados:
- Las proteínas ORC, Cdc45p y MCM son componentes del pre-RC.
- La asociación MCM-origen depende de ORC y Cdc6p.
- Las proteínas MCM y Cdc45p se disocian del ADN de origen durante la fase S y se asocian con el ADN no de origen, similar a la ADN polimerasa epsilon.
Conclusiones:
- Componentes proteicos identificados del pre-RC y un nuevo complejo de replicación (RC) en la transición G1/S.
- Las proteínas MCM y Cdc45p probablemente se muevan con las horquillas de replicación eucariota después de la iniciación.
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