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Las quinasas MAP con distintas funciones inhibidoras imparten especificidad de señalización durante la diferenciación

H D Madhani¹, C A Styles, G R Fink

¹Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.

|December 11, 1997

Resumen

El estudio revela roles distintos para Fus3 y Kss1 kinasas de proteínas activadas por mitógeno (MAPK) en la levadura. Kss1 controla la filamentación, mientras que Fus3 regula el apareamiento, y ambos poseen funciones inhibidoras únicas para evitar el cruce de vías.

Área de la Ciencia:

Las vías de señalización celular.
Biología molecular La biología molecular.
Genética de la levadura genética de la levadura.

Sus antecedentes:

El crecimiento invasivo filamentoso en *Saccharomyces cerevisiae* (levadura) depende de la cascada de señalización de la proteína quinasa activada por mitógeno (MAPK).
Esta cascada comparte componentes con la vía de respuesta de feromonas de apareamiento, lo que sugiere una posible superposición o cruce entre estos procesos celulares.

Objetivo del estudio:

Para aclarar los roles específicos de diferentes MAPK en distintos procesos celulares como el apareamiento y la filamentación.
Investigar las funciones dependientes de la quinasa e independientes de estas MAPKs.
Comprender cómo se regula y previene la intersección de vías.

Principales métodos:

Análisis comparativo de la función de MAPK en *S. cerevisiae*.
Manipulación genética para estudiar las funciones de Fus3 y Kss1 MAPKs.
Investigación de las interacciones proteína-proteína y la regulación transcripcional.

Principales resultados:

Fus3 MAPK está involucrado principalmente en el apareamiento, mientras que Kss1 MAPK regula la filamentación y la invasión.
Tanto Kss1 como Fus3 exhiben funciones inhibidoras independientes de la quinasa.
Kss1 inhibe directamente la vía de filamentación al interactuar con el factor de transcripción Ste12.
Fus3 previene la activación inapropiada de la invasión por la vía de respuesta de feromonas.
La pérdida de Fus3 conduce a la crosstalk, donde la feromona de apareamiento activa genes específicos de la filamentación a través de Kss1.1.

Conclusiones:

Las vías de apareamiento y filamentación en *S. cerevisiae* utilizan MAPKs distintas (Fus3 y Kss1, respectivamente).
Las funciones independientes de la quinasa de Fus3 y Kss1 son cruciales para mantener la especificidad de la vía y prevenir la señalización aberrante.
La comprensión de estos distintos roles y mecanismos reguladores es clave para comprender las decisiones del destino de las células de levadura y el crecimiento invasivo.

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