Regulación de la transcripción mediante el inicio de la concentración de NTP: síntesis de rRNA en bacterias

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T Gaal¹, M S Bartlett, W Ross

¹Department of Bacteriology, University of Wisconsin, 1550 Linden Drive, Madison, WI 53706, USA.

Science (New York, N.Y.)

|February 12, 1998

Resumen

Los promotores de ARN ribosomal (rrn P1) de Escherichia coli necesitan altas concentraciones de nucleósido trifosfato (NTP) para iniciar la transcripción debido a los complejos de ARN polimerasa inestables. Este control dependiente de la NTP explica cómo las bacterias regulan la síntesis de ribosomas en función de la tasa de crecimiento.

Área de la Ciencia:

Biología Molecular Biología Molecular
Genética La genética.
La bioquímica es la bioquímica.

Sus antecedentes:

La secuencia promotora influye en la formación del complejo de ARN polimerasa y en la iniciación de la transcripción.
Los promotores de ARN ribosómico (rrn P1) de Escherichia coli requieren altas concentraciones de nucleósido trifosfato (NTP) de iniciación.

Objetivo del estudio:

Para explicar por qué los promotores de rrn P1 necesitan altas concentraciones de NTP para la transcripción.
Aclarar el mecanismo molecular detrás de la regulación dependiente de la tasa de crecimiento de la síntesis de ribosomas.

Principales métodos:

Análisis de la estabilidad del complejo ARN polimerasa-promotor.
Investigando el papel de la iniciación de NTP (ATP o GTP) en la estabilización de complejos abiertos.
Correlación de las concentraciones de NTP con las tasas de crecimiento bacteriano.

Principales resultados:

rrn Los promotores de P1 forman complejos de corta duración con la ARN polimerasa.
Las altas concentraciones de NTP iniciadoras (ATP o GTP) estabilizan estos complejos.
Las concentraciones de ATP y GTP aumentan con la tasa de crecimiento bacteriano.

Conclusiones:

La concentración de NTP es un factor clave en el control de la actividad del promotor de rrn P1.
Esto proporciona una base molecular para la regulación dependiente de la tasa de crecimiento de la síntesis de ribosomas y la homeostasis.

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