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Determining heat and mechanical pain threshold in inflamed skin of human subjects
Published on: January 14, 2009
Las taquicininas aferentes primarias son necesarias para experimentar dolor moderado a intenso
Y Q Cao1, P W Mantyh, E J Carlson
1Department of Anatomy, W.M. Keck Foundation Center for Integrative Neuroscience, University of California San Francisco, 94143-0452, USA.
Nature
|April 16, 1998
Resumen
La sustancia P, un péptido que se encuentra en las neuronas sensibles al dolor, es crucial para la percepción del dolor de moderado a intenso. La alteración de su gen redujo significativamente las respuestas al dolor y la inflamación neurogénica en ratones.
Área de la Ciencia:
- La neurociencia es la neurociencia.
- Investigación del dolor Investigación del dolor.
- Biología Molecular Biología Molecular
Sus antecedentes:
- El glutamato es un neurotransmisor excitatorio involucrado en la señalización del dolor.
- El papel de la sustancia P en la transmisión del dolor no se entiende completamente.
- Los aferentes primarios que responden a estímulos dolorosos contienen tanto glutamato como la sustancia P.
Objetivo del estudio:
- Investigar el papel de la sustancia P en la percepción del dolor y la inflamación neurogénica.
- Para determinar si la sustancia P es esencial para la señalización del dolor en el cuerno dorsal.
Principales métodos:
- Disrupción del gen de la preprotaquicinina A del ratón (PPT-A), que codifica la sustancia P y la neurokinina A.
- Evaluación de las respuestas conductuales a estímulos dolorosos ligeramente dolorosos y de moderados a intensos.
- Evaluación de la inflamación neurogénica en ratones mutantes.
Principales resultados:
- Los ratones que carecían de la sustancia P (noqueo PPT-A) mostraron respuestas intactas al dolor leve.
- Las respuestas conductuales de dolor moderado a intenso se redujeron significativamente en ratones mutantes.
- La inflamación neurogénica estuvo casi ausente en los ratones knockout de PPT-A.
Conclusiones:
- La liberación de taquicinina de los nociceptores aferentes primarios es esencial para el dolor moderado a intenso.
- La sustancia P juega un papel importante en la señalización del dolor y la inflamación neurogénica.
- El glutamato puede mediar el dolor leve, mientras que las taquicininas son críticas para el dolor más intenso.
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