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Published on: December 19, 2018
El estrógeno modula la expresión génica del receptor AT1 in vitro e in vivo
G Nickenig1, A T Bäumer, C Grohè
1Klinik III für Innere Medizin, Universität zu Köln, Germany. georg.nickenig@uni-koeln.de
Circulation
|June 19, 1998
Resumen
La deficiencia de estrógeno aumenta la expresión del receptor AT1, lo que contribuye a la hipertensión y la aterosclerosis. La terapia de reemplazo de estrógenos invierte este efecto, destacando su papel protector en la salud cardiovascular.
Área de la Ciencia:
- Biología Cardiovascular Biología Cardiovascular
- Endocrinología Endocrinología.
- La medicina molecular es una medicina molecular.
Sus antecedentes:
- El receptor de la angiotensina II tipo 1 (AT1) está implicado en la hipertensión y la aterosclerosis.
- La deficiencia de estrógeno está relacionada con un mayor riesgo de enfermedad cardiovascular.
Objetivo del estudio:
- Para investigar el efecto de la deficiencia y el reemplazo de estrógenos en la expresión génica del receptor AT1 en ratas con ovariectomía.
- Para determinar la influencia del estrógeno en la expresión del receptor AT1 en las células del músculo liso vascular.
Principales métodos:
- Se estudiaron ratas con ovariectomía con y sin reemplazo de estrógeno.
- Se evaluó la vasoconstricción inducida por la angiotensina II, los niveles de ARNm de los receptores AT1 (qPCR, Northern blotting) y la densidad de los receptores AT1 (conexión a los radioligandos).
- También se realizaron experimentos en células musculares lisas vasculares cultivadas.
Principales resultados:
- La deficiencia de estrógeno aumentó significativamente la densidad del receptor AT1 (160%) y los niveles de ARNm (187%) en aortas de rata.
- La terapia de reemplazo de estrógenos normalizó la sobreexpresión del receptor AT1.
- El estradiol regula a la baja el ARNm del receptor AT1 y la densidad en las células musculares lisas vasculares cultivadas.
Conclusiones:
- La deficiencia de estrógeno regula la expresión del receptor AT1, lo que podría explicar su asociación con la hipertensión y la aterosclerosis.
- El estrógeno ejerce un efecto supresor directo sobre la expresión del receptor AT1 en las células del músculo liso vascular.
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