Video Experimental Relacionado
Updated: Jul 22, 2026
09:32
Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
Published on: June 15, 2017
Dishevelled activa la JNK y discrimina entre las vías de la JNK en polaridad plana y la señalización sin alas
M Boutros1, N Paricio, D I Strutt
1European Molecular Biology Laboratory, Developmental Biology Programme, Heidelberg, Germany.
Cell
|July 23, 1998
Resumen
La proteína desarticulada activa las vías de señalización JNK. Diferentes señales Wnt/Frizzled utilizan distintos dominios Dishevelled para regular la polaridad plana y la morfogénesis.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- La señalización celular de las células.
- Genética molecular genética molecular.
Sus antecedentes:
- Las proteínas frizzled actúan como receptores para las moléculas de señalización Wnt.
- En Drosophila, las proteínas Frizzled están involucradas en distintos procesos de desarrollo como la polaridad plana y la morfogénesis.
- Dishevelled es una proteína crucial requerida en ambas vías de señalización Wnt/Frizzled.
Objetivo del estudio:
- Investigar el papel de Dishevelled en las vías de señalización de Wnt/Frizzled.
- Para determinar cómo Dishevelled activa cascadas intracelulares como la señalización JNK.
- Para aclarar los requisitos específicos de dominio de Dishevelled en diferentes procesos mediados por Wnt/Frizzled.
Principales métodos:
- Análisis genéticos en Drosophila.
- Estudios de la sobreexpresión.
- Análisis de rescate de los defectos del desarrollo.
- Análisis de mutaciones específicas del alelo. análisis de mutaciones específicas del alelo.
Principales resultados:
- Se demostró que el desordenado activa las cascadas de señalización de JNK.
- Se requieren dominios proteicos distintos de Dishevelled para su función en la polaridad plana y la morfogénesis.
- El dominio DEP C-terminal de Dishevelled es esencial para rescatar los defectos de polaridad plana e inducir la señalización JNK.
- Se encontró que un alelo específico, dsh1, que afecta la polaridad plana, estaba mutado en el dominio DEP.
Conclusiones:
- Diferentes señales Wnt/Frizzled activan distintas vías intracelulares.
- Dishevelled actúa como un transductor de señal, discriminando entre señales Wnt / Frizzled a través de interacciones específicas de dominio.
- El dominio DEP de Dishevelled juega un papel crítico en la mediación de la transducción de señales Wnt / Frizzled para la polaridad plana y la activación de JNK.
Más Videos Relacionados
05:48Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
Published on: January 2, 2018
07:26Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
Published on: April 1, 2022
Videos de Conceptos Relacionados
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
01:07MAPK Signaling Cascades
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
01:20The JAK-STAT Signaling Pathway
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
01:16TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
01:25cAMP-dependent Protein Kinase Pathways
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...