Video Experimental Relacionado
Updated: Jul 15, 2026
15:42
Double Whole Mount in situ Hybridization of Early Chick Embryos
Published on: October 27, 2008
Pitx2 participa en la fase tardía de la vía que controla la asimetría izquierda-derecha
M E Piedra1, J M Icardo, M Albajar
1Departamento de Anatomía y Biología Celular, Facultad de Medicina, Universidad de Cantabria, Santander, Spain.
Cell
|August 26, 1998
Resumen
La expresión del gen Pitx2 es asimétrica en el desarrollo de los embriones, y su regulación por Shh y nodal sugiere un papel en el control de la asimetría izquierda-derecha del cuerpo.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Genética La genética.
- Biología molecular La biología molecular.
Sus antecedentes:
- Pitx2 es un gen homeobox implicado en el desarrollo embrionario.
- La expresión génica asimétrica es crucial para establecer los ejes izquierdo-derecho del cuerpo.
- Estudios previos vincularon Pitx2 con anomalías del sitio.
Objetivo del estudio:
- Para investigar el papel de Pitx2 en el control genético de la lateralidad.
- Explorar la regulación de la expresión de Pitx2 durante el desarrollo embrionario.
Principales métodos:
- Análisis de los patrones de expresión de Pitx2 en embriones de pollo y ratón.
- Estudio de la mutación iv del ratón y su efecto en la expresión de Pitx2.
- Experimentos de expresión errónea para determinar las relaciones regulatorias.
Principales resultados:
- Pitx2 exhibe expresión asimétrica en el mesodermo de la placa lateral izquierda y sus derivados.
- Las alteraciones en la expresión de Pitx2 se correlacionan con los defectos del sitio en la mutación del ratón iv.
- Sonic hedgehog (Shh) y las vías de señalización nodal regulan positivamente la expresión de Pitx2.
Conclusiones:
- Pitx2 juega un papel importante en las últimas etapas de la red reguladora de genes que controla la asimetría del cuerpo.
- Shh y nodal son reguladores principales aguas arriba de Pitx2 en la determinación de la lateralidad.
Videos de Conceptos Relacionados
01:33Pleiotropy
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
01:58X-Inactivation
The human X chromosome contains over ten times the number of genes as in the Y chromosome. Since males have only one X chromosome, and females have two, one might expect females to produce twice as many of the proteins, with undesirable results.
03:17Inheritance of Chromatin Structures
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
01:58X-inactivation
The human X chromosome contains over ten times the number of genes as in the Y chromosome. Since males have only one X chromosome, and females have two, one might expect females to produce twice as many of the proteins, with undesirable results.
02:24Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...