Video Experimental Relacionado
Updated: Aug 9, 2026
10:05
Improved Generation of Induced Cardiomyocytes Using a Polycistronic Construct Expressing Optimal Ratio of Gata4, Mef2c and Tbx5
Published on: November 13, 2015
La activación de gp130 transduce las señales hipertróficas a través de STAT3 en los miocitos cardíacos
1Department of Medicine III, Osaka University Medical School, Suita, Japan.
Circulation
|August 26, 1998
Resumen
La vía STAT3, aguas abajo de gp130, promueve la hipertrofia de los miocitos cardíacos. Este estudio investigó el STAT3.
Área de la Ciencia:
- Biología Cardiovascular Biología Cardiovascular
- Biología celular molecular Biología celular molecular
- Transducción de señales Transducción de señales.
Sus antecedentes:
- gp130, un transductor de señales clave para las citoquinas relacionadas con la IL-6, está presente en los miocitos cardíacos.
- La activación de gp130 en los miocitos cardíacos está relacionada con la hipertrofia miocárdica.
- Existen dos vías principales aguas abajo: JAK/STAT y MAPK.
Objetivo del estudio:
- Investigar el papel crítico de la vía de Janus kinase / transductor de señal y activador de la transcripción (JAK / STAT), específicamente STAT3, en la hipertrofia de miocitos cardíacos dependientes de gp130.
- Para aclarar la participación de STAT3 en la mediación de la señalización de la hipertrofia en respuesta al factor inhibidor de la leucemia (LIF).
Principales métodos:
- Se utilizaron vectores de adenovirus para transfectar el tipo salvaje (AD / WT) o el tipo mutado (AD / DN) STAT3 cDNA en miocitos cardíacos murinos cultivados.
- Las células fueron estimuladas con el factor inhibidor de la leucemia (LIF).
- Se midió la expresión de los ARNm de c-fos y el factor natriurético auricular (FNA) y la síntesis de proteínas (incorporación de leucina [3H]); también se evaluó la actividad de MAPK.
Principales resultados:
- La activación de STAT3 aumentó significativamente la inducción de los ARNm c-fos y ANF y la síntesis de proteínas.
- Inhibición de la expresión de ARNm STAT3 atenuado c-fos inducido por LIF y ANF y síntesis de proteínas.
- No se observaron diferencias significativas en la actividad de MAPK entre los grupos, lo que sugiere un papel específico para STAT3.
Conclusiones:
- La vía de señalización STAT3-dependiente aguas abajo de gp130 juega un papel crítico en la promoción de la hipertrofia de los miocitos cardíacos.
- Esta vía es activada por el factor inhibidor de la leucemia (LIF) y contribuye a la remodelación cardíaca.
Más Videos Relacionados
Videos de Conceptos Relacionados
01:22Amplifying Signals via Enzymatic Cascade
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...
01:20The JAK-STAT Signaling Pathway
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
01:16TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
01:22Activation and Inactivation of G Proteins
Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high affinity and are together...
01:29Cardiomyopathy III: Hypertrophic Cardiomyopathy
Hypertrophic cardiomyopathy, or HCM, is an autosomal dominant genetic disorder characterized by asymmetric left ventricular hypertrophy without ventricular dilation. It is more common in men and is typically diagnosed in young, athletic adults.EtiologyHCM is primarily genetic and is caused by mutations in genes encoding sarcomeric proteins. Researchers have identified over 1400 mutations across at least 11 different genes. Among these, the most frequently occurring mutations are found in the...
01:26Cellular Adaptation II: Hypertrophy
Hypertrophy is the increase in the size of individual cells, resulting in the enlargement of a tissue or organ. Unlike hyperplasia, which involves an increase in cell number, hypertrophy is characterized by an increase in cell volume. This process often occurs in response to higher functional demand or hormonal stimulation, leading to the production of more structural proteins and organelles, thereby enhancing the cells' work capacity.There are two primary types of hypertrophy: physiological...