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El mantenimiento de la LTP de fase tardía se acompaña de un aumento dependiente de PKA en la síntesis del receptor
A Nayak1, D J Zastrow, R Lickteig
1Neuroscience Program, University of Colorado Health Sciences Center, Denver 80262, USA.
Nature
|August 26, 1998
Resumen
La LTP de fase tardía, crucial para la memoria, no requiere transcripción de genes presinápticos. En cambio, los mecanismos postsinápticos que involucran la síntesis de los receptores AMPA, impulsados por la transcripción dependiente de PKA, mantienen esta forma crucial de plasticidad sináptica.
Área de la Ciencia:
- La neurociencia es la neurociencia.
- La plasticidad sináptica.
Sus antecedentes:
- La potenciación a largo plazo (LTP) es un mecanismo clave para el aprendizaje y la memoria.
- La LTP de fase tardía (3-5 horas después de la estimulación) depende de la transcripción, la síntesis de proteínas y la proteína quinasa A (PKA).
- El papel de la transcripción génica presináptica frente a la postsináptica en la fase tardía de la LTP sigue sin estar claro.
Objetivo del estudio:
- Investigar si la transcripción génica presináptica es necesaria para mantener la fase tardía de la LTP.
- Para aclarar los mecanismos postsinápticos involucrados en el mantenimiento de la fase tardía de la LTP.
Principales métodos:
- Utilizó mini-rebanadas CA1 del hipocampo de rata con cuerpos celulares presinápticos eliminados.
- Investigó la síntesis de subunidades del receptor AMPA después de la inducción de LTP.
- Examinó los efectos del PKA y los inhibidores de la transcripción en la síntesis del receptor AMPA.
Principales resultados:
- La LTP de fase tardía se indujo con éxito en mini-retallas que carecían de cuerpos celulares presinápticos, lo que indica que no se requiere la transcripción presináptica.
- La síntesis de subunidades del receptor AMPA aumentó significativamente tres horas después de la inducción de LTP.
- Los inhibidores de la PKA y la transcripción bloquearon este aumento en la síntesis del receptor AMPA.
Conclusiones:
- La transcripción génica presináptica no es esencial para mantener la fase tardía de la LTP.
- Un mecanismo postsináptico que implica un aumento de la síntesis de los receptores AMPA, regulado por la transcripción génica dependiente de PKA, subyace al mantenimiento de la LTP en fase tardía.
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