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La inducción del mesodermo mediada por FGF involucra a la quinasa de la familia Src Laloo
D C Weinstein1, J Marden, F Carnevali
1Department of Molecular Vertebrate Embryology, The Rockefeller University, New York, New York 10021, USA.
Nature
|September 11, 1998
Resumen
Los científicos descubrieron una nueva quinasa relacionada con Src, laloo, crucial para la inducción del mesodermo durante la embriogénesis de Xenopus. Laloo Laloo es el nombre de su hija.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Biología Molecular Biología Molecular
- La señalización celular de las células.
Sus antecedentes:
- El desarrollo embrionario se basa en señales inductivas para establecer el plan corporal.
- En Xenopus laevis, la inducción del mesodermo está mediada por factores del polo vegetal, que involucran las vías de señalización TGF-beta y FGF.
Objetivo del estudio:
- Identificar y caracterizar nuevos participantes en la cascada de transducción de señales de la inducción del mesodermo.
- Para investigar el papel de un miembro de la familia de genes relacionados con Src en la embriogénesis de vertebrados.
Principales métodos:
- Sobreexpresión de laloo en embriones de Xenopus.
- Análisis de la inducción del mesodermo en explantes ectodérmicos de Xenopus.
- Utilizando mutantes dominantes inhibidores para bloquear las vías de señalización.
Principales resultados:
- La sobreexpresión de laloo condujo a estructuras posteriores ectópicas, incluido el tejido axial.
- Laloo indujo mesodermo en explantes ectodérmicos, un proceso inhibido al interrumpir la señalización de FGF.
- Un mutante Laloo inhibidor dominante bloqueó la inducción del mesodermo inducida por FGF y causó truncamientos posteriores.
Conclusiones:
- Laloo es una nueva quinasa relacionada con Src involucrada en la inducción del mesodermo de los vertebrados.
- Este estudio proporciona la primera evidencia que vincula las cinasas relacionadas con Src a la inducción del mesodermo durante el desarrollo de los vertebrados.
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