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A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
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Una nueva proteína adaptadora orquesta el patrón del receptor y la polaridad citoesquelética en los contactos de
M L Dustin1, M W Olszowy, A D Holdorf
1Department of Pathology and Center for Immunology, Washington University School of Medicine, Saint Louis, Missouri 63110, USA.
Cell
|September 19, 1998
Resumen
El reconocimiento de las células T implica uniones celulares especializadas. El compromiso de CD2 desencadena la segregación de proteínas, el agrupamiento y los cambios citoesqueléticos, mediados por la nueva proteína CD2AP que une los receptores al citoesqueleto.
Área de la Ciencia:
- Inmunología Inmunología.
- Biología celular Biología celular.
- La bioquímica es la bioquímica.
Sus antecedentes:
- El reconocimiento de antígenos por parte de las células T requiere uniones especializadas entre células.
- El reclutamiento y la exclusión de proteínas en estas uniones son críticos pero mal entendidos.
- La molécula de adhesión CD2 juega un papel en las interacciones de células T-APC.
Objetivo del estudio:
- Investigar los mecanismos que regulan la organización de las proteínas en la interfaz T-APC.
- Para identificar las proteínas involucradas en la señalización mediada por CD2 y los reordenamientos citoesqueléticos.
Principales métodos:
- Estudió los efectos de la participación del ligando CD2 en la distribución de proteínas y la morfología celular.
- Investigó el papel del dominio citoplasmático CD2 en estos procesos.
- Identificó y caracterizó una nueva proteína que contiene SH3, CD2AP.
Principales resultados:
- El compromiso de CD2 inició la segregación de proteínas, el agrupamiento de CD2 y la polarización citoesquelética.
- La segregación de proteínas fue independiente del dominio citoplasmático CD2.
- El agrupamiento de CD2 y la polarización citosquelética requirieron la interacción de CD2AP con el dominio citoplasmático de CD2.
Conclusiones:
- CD2AP es una nueva proteína esencial para la formación de uniones de células T-APC mediadas por CD2.
- CD2AP enlaza los receptores de adhesión con el citoesqueleto, lo que facilita el patrón del receptor.
- Comprender la función de CD2AP proporciona información sobre la activación de las células T y la formación de sinapsis inmunes.
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