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Apaf1 (homólogo de CED-4) regula la muerte celular programada en el desarrollo de mamíferos
F Cecconi1, G Alvarez-Bolado, B I Meyer
1Department of Molecular Cell Biology, Max Planck Institute of Biophysical Chemistry, Göttingen, Germany.
Cell
|September 30, 1998
Resumen
El factor de activación de la proteasa apoptótica 1 (Apaf1) es crucial para el desarrollo de los mamíferos. Su ausencia conduce a la letalidad embrionaria y a graves defectos de desarrollo debido a la muerte celular programada alterada.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Biología celular Biología celular.
- Genética La genética.
Sus antecedentes:
- El factor de activación de la proteasa apoptótica 1 (Apaf1) es un componente clave de la vía apoptótica intrínseca.
- Interviene en la activación de la caspasa 9 (CASP9) y posteriormente de la caspasa 3 (CASP3).
- El papel de Apaf1 en el desarrollo embrionario de mamíferos y la muerte celular programada no se entiende completamente.
Objetivo del estudio:
- Investigar la función de Apaf1 en el desarrollo embrionario de mamíferos.
- Para determinar el papel de Apaf1 en la muerte celular programada durante la embriogénesis.
Principales métodos:
- Generación de una trampa génica alelo nulo para el murino Apaf1.1.
- Análisis fenotípico de embriones homocigotos mutantes Apaf1.
- In situ inmunodetección de la activación de la caspasa 3.
Principales resultados:
- Los mutantes homocigotos Apaf1 exhiben letalidad embrionaria al día 16.5.
- Los mutantes muestran severas malformaciones craneofaciales, crecimiento excesivo del cerebro, redes interdigitales persistentes y anormalidades oculares.
- La deficiencia de Apaf1 da como resultado una respuesta apoptótica reducida en los fibroblastos e impide la activación in vivo de la Caspasa 3.
Conclusiones:
- Apaf1 es esencial para la activación de la caspasa 3 en el cerebro embrionario.
- Apaf1 juega un papel crítico en la regulación de la muerte celular programada del desarrollo en los mamíferos.
- La ausencia de Apaf1 conduce a graves defectos de desarrollo y letalidad embrionaria.
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