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Se requiere un complejo de remodelación de la cromatina relacionado con SWI / SNF, E-RC1, para la regulación

J A Armstrong¹, J J Bieker, B M Emerson

¹Regulatory Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, California 92037, USA.

|October 20, 1998

Resumen

El factor eritroide parecido a Krüppel (EKLF) necesita un complejo de remodelación de la cromatina (E-RC1) para activar el gen beta-globina. Este complejo relacionado con SWI / SNF interactúa selectivamente con las proteínas de unión al ADN para la regulación génica.

Área de la Ciencia:

Biología Molecular Biología Molecular
Regulación genética Reglamento genético.
Dinámica de la cromatina Dinámica de la cromatina Dinámica de la cromatina Dinámica de la cromatina Dinámica de la cromatina Dinámica de la cromatina Dinámica de la cromatina Dinámica de la cromatina Dinámica de la cromatina Dinámica de la cromatina

Sus antecedentes:

El factor eritroide similar a Krüppel (EKLF) es crucial para la expresión específica de la etapa del gen beta-globina humano.
La estructura de la cromatina juega un papel importante en la regulación de la accesibilidad y transcripción de los genes.

Objetivo del estudio:

Para investigar el papel de la remodelación de la cromatina en la activación del gen beta-globina mediada por EKLF.
Para identificar los componentes del complejo de coactivadores requeridos para la función EKLF.

Principales métodos:

Ensayos de transcripción in vitro utilizando plantillas de cromatina.
Caracterización bioquímica del complejo de remodelación-coactivador del EKLF 1 (E-RC1).
Análisis de la interacción de E-RC1 con los factores de transcripción y las proteínas de unión al ADN.

Principales resultados:

EKLF requiere el complejo E-RC1 relacionado con SWI / SNF para crear un promotor activo de beta-globina en la cromatina.
E-RC1 comprende homólogos de las subunidades SWI / SNF de levadura (BRG1, BAF170, BAF155, INI1) y una subunidad única, BAF57.
E-RC1 exhibe selectividad funcional, activando la transcripción dependiente del EKLF pero no la transcripción mediada por el TFE-3.

Conclusiones:

Los miembros de la familia SWI/SNF participan directamente en la activación transcripcional.
Los complejos de remodelación de cromatina pueden regular los subconjuntos de genes a través de interacciones selectivas con las proteínas de unión al ADN.
E-RC1 es un coactivador clave para EKLF en la expresión génica de la beta-globina.

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