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El receptor Msn5 exporta el factor de transcripción fosforilado Pho4 fuera del núcleo
A Kaffman1, N M Rank, E M O'Neill
1Department of Biochemistry and Biophysics, University of California at San Francisco, School of Medicine, 94143-0448, USA.
Nature
|December 16, 1998
Resumen
La fosforilación del factor de transcripción de la levadura Pho4 desencadena su exportación nuclear. Este proceso utiliza el receptor Msn5, revelando un mecanismo clave para controlar la localización y la actividad de las proteínas.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
Sus antecedentes:
- El transporte nuclear-citoplasmático de las proteínas regula su actividad.
- La fosforilación es una modificación post-traducional clave que controla la localización de las proteínas.
- Los mecanismos de la exportación nuclear inducida por la fosforilación no se comprenden completamente.
Objetivo del estudio:
- Investigar la regulación del factor de transcripción de la levadura Pho4 exportación nuclear.
- Aclarar el papel de la fosforilación en el control de la localización de Pho4.
- Identifique el receptor de exportación involucrado en la exportación nuclear de Pho4.
Principales métodos:
- Ensayos bioquímicos in vivo y in vitro.
- Análisis de la fosforilación y localización de proteínas.
- Genética de la levadura y técnicas de biología molecular.
Principales resultados:
- La exportación de Pho4 desde el núcleo se desencadena por fosforilación mediada por el complejo Pho80-Pho85.
- Msn5, un miembro de la familia de las importinas beta, actúa como receptor nuclear de exportación para Pho4.4.
- Msn5 se une al Pho4 fosforilado de una manera dependiente de Ran-GTP.
Conclusiones:
- La fosforilación por Pho80-Pho85 regula directamente la exportación nuclear de Pho4.
- Msn5 media en la exportación dependiente de la fosforilación de Pho4.
- Este estudio revela un mecanismo fundamental para controlar la exportación nuclear de proteínas a través de la fosforilación.
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