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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Updated: May 11, 2026

RNA Catalyst as a Reporter for Screening Drugs against RNA Editing in Trypanosomes
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Published on: July 22, 2014

Edición de los receptores inmunes: revisar y seleccionar.

M C Nussenzweig1

  • 1Laboratory of Molecular Immunology, The Rockefeller University, Howard Hughes Medical Institute, New York, New York 10021, USA.

Cell
|January 6, 1999
PubMed
Resumen

La recombinación genética del receptor de antígeno secundario, o edición del receptor, salva a los linfocitos autorreactivos antes de la selección negativa. Este proceso, crucial en el desarrollo de las células T y B, da forma a las respuestas inmunes y no fue predicho por teorías anteriores.

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Área de la Ciencia:

  • Inmunología Inmunología.
  • Biología Molecular Biología Molecular
  • Biología celular Biología celular.

Sus antecedentes:

  • La recombinación genética del receptor de antígeno secundario ocurre tanto en linfocitos en desarrollo como en linfocitos maduros.
  • La edición de receptores en el desarrollo de los linfocitos implica alterar la especificidad del receptor en respuesta a los autoantígenos.
  • Este proceso salva a las células autorreactivas, impidiendo su eliminación a través de la selección negativa.

Objetivo del estudio:

  • Para explorar el fenómeno de la edición de receptores en los linfocitos.
  • Comprender cómo los cambios en la especificidad de los receptores inducidos por antígenos pueden integrarse en las teorías inmunológicas existentes.
  • Para resaltar la importancia de la recombinación V(D) J en linfocitos maduros.

Principales métodos:

  • El estudio analiza el concepto de edición de receptores basado en los principios inmunológicos existentes y la literatura.
  • Hace referencia a marcos teóricos como la teoría de la selección clonal y las sugerencias de Jerne.
  • El texto implica una revisión y síntesis de los conocimientos sobre el desarrollo y la selección de los linfocitos.

Principales resultados:

  • La edición de receptores convierte las células anti-auto-reactivas en células no auto-reactivas durante el desarrollo de los linfocitos.
  • Este mecanismo salva a los clones autorreactivos antes de que se sometan a una selección negativa.
  • Los cambios inducidos por antígenos en la especificidad de los receptores no se tuvieron en cuenta inicialmente en la teoría de la selección clonal.

Conclusiones:

  • La edición de receptores se puede incorporar a la teoría de la selección clonal considerando una etapa de desarrollo específica.
  • Si bien se sabe menos sobre la recombinación V(D) J en linfocitos maduros, es probable que influya en las respuestas inmunes.
  • La selección de receptores inducida por antígenos juega un papel importante en la configuración de la respuesta inmune general.