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El procollageno atraviesa la pila de Golgi sin salir del lumen de las cisternas: evidencia de maduración cisterna
L Bonfanti1, A A Mironov, J A Martínez-Menárguez
1Istituto di Ricerche Farmacologiche Mario Negri, Consorzio Mario Negri Sud, Department of Cell Biology and Oncology, Chieti, Italy.
Cell
|January 6, 1999
Resumen
El procollageno de tipo I (PC) recién sintetizado forma grandes estructuras que se mueven a través del aparato de Golgi. Este transporte de carga supermolecular sugiere un nuevo mecanismo más allá del transporte convencional de vesículas, que involucra la maduración de la cisterna de Golgi.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
Sus antecedentes:
- El procollageno de tipo I (PC) recién sintetizado oligomeriza en rígidas hélices triples en forma de varilla dentro del retículo endoplasmático.
- Estos complejos son transportados al aparato de Golgi, donde forman grandes agregados densos en electrones.
Objetivo del estudio:
- Para monitorear el transporte de procollageno tipo I (PC) a lo largo de la vía secretora.
- Para investigar el mecanismo por el cual las cargas supermoleculares atraviesan el complejo de Golgi.
Principales métodos:
- Monitoreo del movimiento de PC dentro del retículo endoplasmático y el aparato de Golgi.
- Analizando la morfología de las estructuras que contienen PC durante el transporte.
Principales resultados:
- El procollageno tipo I (PC) se mueve a través de las pilas de Golgi mientras permanece dentro del lumen de Golgi cisternae.
- Durante el transporte de ER a Golgi, se observa PC en estructuras tubulares-saculares de más de 300 nm de longitud.
- Las cargas supermoleculares como el PC no parecen utilizar el transporte convencional mediado por vesículas a través del Golgo.
Conclusiones:
- El transporte de procollageno tipo I (PC) a través del complejo de Golgi se produce a través de un mecanismo no vesicular.
- Este transporte probablemente implica la maduración progresiva de las cisternas de Golgi, lo que facilita el movimiento de grandes conjuntos moleculares.
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