新しい分泌されたタンパク質がWntタンパク質に結合し,その活動を抑制します

J C Hsieh¹, L Kodjabachian, M L Rebbert

¹Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

Nature

|April 14, 1999

まとめ

Wnt阻害因子-1 (WIF-1) は,Wntタンパク質に結合し,その発達信号伝達を阻害する新しい分泌タンパク質です. WIF-1は,WIF-1に設定されている.

科学分野:

発達生物学発達生物学について
分子シグナリング
バイオケミストリーバイオケミストリー

背景:

Wntタンパク質は,様々な発達プロセスに関与する重要な細胞外信号伝達分子です.
Wnt信号調節の理解は,正常な発達と疾患の病理学の両方にとって不可欠です.

研究の目的:

Wnt信号伝達の新しいレギュレータを特定し,特徴づけること.
胚の発達におけるWnt阻害因子-1 (WIF-1) の機能を調査する.

主な方法:

Wnt阻害因子-1 (WIF-1),分泌されるタンパク質について説明します.
クセノプスとゼブラフィッシュの胚のWIF-1発現パターンを分析した.
WIF-1過剰発現がXenopus somitogenesisに与える影響を研究した.
Wntタンパク質とインビトロ結合測定を行った.

主要な成果:

WIF-1は脊椎動物全体に保存され,発達中に複雑な発現パターンを表しています.
Xenopus胚におけるWIF-1の過剰発現は,ソミトゲネシスを破壊した.
WIF-1は,Drosophila WinglessとXenopus Wnt8のタンパク質に直接結合する.

結論:

WIF-1は,Wnt信号伝達の細胞外阻害剤として作用する.
Wntタンパク質は,様々な細胞外阻害物質と相互作用して,信号伝達を調節する.
これらの発見は,開発中のWnt活動の微調整を理解するのに役立ちます.

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