ドロソフィラのノッチの活動と核へのアクセスにはプレセニリンが必要です
1Department of Genetics and Development, Howard Hughes Medical Institute, Columbia University, College of Physicians and Surgeons, New York, New York 10032, USA. struhl@cuccfa.ccc.columbia
Nature
|April 17, 1999
まとめ
プレセニリンは,ノッチ信号伝達,遺伝子転写の調節に不可欠です. ドロソフィラ・プレセニリンのヌル変異は,ノッチ・シグナリングを廃止し,細胞内領域の核の侵入を防ぐ.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- 神経科学は神経科学である.
背景:
- プレセニリンは,β-アミロイド前駆体タンパク質の処理を通じてアルツハイマー病の病原性に関与する膜タンパク質です.
- プレセニリンは,LIN-12/Notchトランスメブラン受容体の活性を調節することが知られている.
- ノッチ受容体の細胞内領域は,リガンド誘発処理後に遺伝子発現を制御するために核に転位する.
研究 の 目的:
- ノッチ信号伝導におけるドロソフィラプレセニリンの役割を調査する.
- ノッチ細胞内領域の核転移にプレセニリンが必要かどうかを判断する.
- プレセニリンがノッチ受容体の活性化に影響を与えるメカニズムを解明する.
主な方法:
- ドロソフィラ・プレセニリン遺伝子のゼロ変異を利用した.
- 評価されたノッチ信号伝達経路.
- ノッチ細胞内ドメインの核エントリを調べた.
- ノッチ細胞内ドメインのタンパク質解離を調査した.
主要な成果:
- ドロソフィラ・プレセニリンのゼロ変異は,ノッチ信号伝導を完全に廃止しました.
- プレセニリンの欠乏は,ノッチの細胞内領域が核に入ることを妨げました.
- プレセニリンは,ノッチ細胞内領域のリガンド誘発のタンパク質解離に不可欠であることが判明しました.
結論:
- プレセニリンは,ノッチ信号伝達を促進する上で重要な役割を果たします.
- ノッチ細胞内ドメインのタンパク質分解処理と,その後の核転位は,プレセニリンの機能に依存しています.
- これらの発見は,ノッチ経路経由による遺伝子転写の調節におけるプレセニリンの重要性を強調しています.
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関連する概念動画
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...