関連する実験動画

Updated: Jul 16, 2026

Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining

Analysis of Simian Immunodeficiency Virus-specific CD8⁺ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining

Published on: December 23, 2016

SHIVを使用したCCR5またはCXCR4に感染したレサス・マカクの明確な病原性シーケラ

J M Harouse¹, A Gettie, R C Tan

¹Aaron Diamond AIDS Research Center, The Rockefeller University, 455 First Avenue, 7th Floor, New York, NY 10016, USA.

Science (New York, N.Y.)

|April 30, 1999

まとめ

Simian-human immunodeficiency virus (SHIV) のモデルは,封筒タンパク質がHIV-1の病原性に影響する方法を明らかにしています. 同受容体使用 (CCR5対CXCR4) は,マカカの異なるCD4+T細胞喪失パターンを決定し,ワクチン開発に影響を与えます.

科学分野:

ウイルス学ウイルス学ウイルス学
免疫学免疫学とは
病原発生研究病原発生研究

背景:

マカクの類人猿-ヒト免疫不全ウイルス (SHIV) 感染は,ヒト免疫不全ウイルス1型 (HIV-1) の病原性を研究するための重要なインビボモデルとして機能しています.
HIV-1封筒タンパク質とウイルス共受容体の役割を理解することは,効果的なワクチンや治療薬の開発に不可欠です.

研究の目的:

マカクのCCR5 (R5) 特定SHIVSF162PとCXCR4 (X4) 特定SHIVSF33A.2との感染症の病原性アウトカムを比較する.
ウイルス共受容体トロピズムが,腸内および外周部におけるCD4+T細胞減少に及ぼす影響を明らかにする.

主な方法:

2つの異なるSHIV株であるSHIVSF162P (R5-熱帯) とSHIVSF33A.2 (X4-熱帯) でマカクの感染.
ウイルスの複製レベルをモニタリングし,腸および周辺組織の両方のCD4+T細胞集団を評価します.
同受容体利用に基づく病原性アウトカムの比較分析.

主要な成果:

R5とX4の両方のSHIV感染は,同様のウイルスの複製レベルを示した.
SHIVSF162P (R5) 感染症は,腸内CD4+T細胞の有意な損失をもたらし,その後は周辺の枯渇を引き起こした.
SHIVSF33A.2 (X4) 感染症は,同様の腸への影響なしに,深い周辺のCD4+T細胞損失を引き起こしました.

結論:

同受容体利用 (CCR5対CXCR4) は,HIV-1の病原性およびCD4+T細胞トロピズムを決定的に影響する.
このSHIVマカクのモデルでは,HIV-1エンベロップタンパク質の機能に関連する異なる病原性プロフィールを効果的に再現しています.
発見は,HIV-1ワクチンおよび封筒タンパク質を標的とした治療法の臨床前評価のためのこのモデルの有用性を支持しています.

さらに関連する動画

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4⁺ T Cells Isolated from Rhesus macaques

Published on: September 25, 2018

Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Humanized NOD/SCID/IL2rγ^null (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Published on: January 7, 2019

関連する概念動画

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...

Cytomegalovirus Disease

Cytomegalovirus Disease

Cytomegalovirus (CMV) disease is caused by human cytomegalovirus, a double-stranded DNA virus of the Herpesviridae family. While primary CMV infection is often asymptomatic in immunocompetent individuals, the virus can cause severe disease in neonates and immunocompromised patients. CMV is the most common cause of congenital viral infection in the United States, and a major pathogen in solid organ and hematopoietic stem cell transplant recipients.CMV is transmitted via bodily fluids, sexual...