核受容体によるリガンド依存転写活性化には,DRIP複合体が必要です
C Rachez1, B D Lemon, Z Suldan
1Cell Biology Program, Memorial Sloan-Kettering Cancer Center, Graduate School of Medical Sciences, Cornell University, New York, New York 10021, USA.
Nature
|May 11, 1999
まとめ
研究者らは,ビタミンD受容体 (VDR) 遺伝子転写に不可欠な複合体を構成する,DRIPと呼ばれる13のタンパク質を特定しました. このDRIP複合体は,ホルモン依存遺伝子の活性化に不可欠であり,異なる転写活性化剤の間で共有されたコファクターを強調しています.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- エンドクリノロジー エンドクリノロジー
背景:
- 核受容体は,リポフィルリンガンドに反応して遺伝子転写を調節する.
- リガンド結合は構成変化を誘導し,トランザクティベーションのための受容体-共因子相互作用を可能にします.
- 以前特定されたDRIP複合体は,ビタミンD受容体 (VDR) と相互作用する.
研究 の 目的:
- DRIPコファクター複合体の構成要素を特定するために.
- ホルモン依存トランザクティベーションにおけるDRIP複合体の機能を明らかにする.
- DRIPsと他のコファクター複合体との関係を調査する.
主な方法:
- タンパク質の識別と特徴付け.
- 細胞フリーシステムを用いたインビトロ転写アッセイ.
- クロマチンベースの転写アッセイ.
主要な成果:
- 13のDRIPが特定され,新しいコファクター複合体を構成しました.
- DRIP複合体は,VDR媒介によるクロマチンのホルモン依存トランザクティベーションにおいて中心的な役割を果たします.
- DRIP/ARCサブユニットは,他のコファクター複合体 (CRSP,NAT,SMCC,Mediator) と共有されています.
結論:
- DRIP複合体は,VDR媒介の遺伝子転写に不可欠である.
- 核受容体リガンドは,DRIPのようなコファクター複合体を募集することによって機能する可能性があります.
- 異なった転写活性化剤は,共有のコファクター複合体を利用することがあり,これは保存された規制メカニズムを示している.
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