複合コアクティベーターARCは,染色体主導の転写活性化を媒介する
A M Näär1, P A Beaurang, S Zhou
1Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720, USA.
Nature
|May 11, 1999
まとめ
アクティベーター・リクルート・コファクター (ARC) は,トランスクリプション・アクティベーターと相互作用することで,遺伝子の活性化を高めます. この大きな共同活性化器複合体は,おそらくDRIPと同一であり,真核生物における遺伝子発現の調節に不可欠である.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオケミストリー バイオケミストリー
背景:
- ユカリオットの遺伝子の活性化には,複雑なメカニズムとDNA結合タンパク質が含まれています.
- クロマチンの包装には,遺伝子調節のための特殊なクロマチンのターゲティング活動が必要です.
- 人間の細胞は,クロマチンの選択性遺伝子活性化のために,マルチサブユニットコファクターを使用します.
研究 の 目的:
- アクティベーター・リクルート・コファクター (ARC) の機能と組成を調べる.
- 様々な転写活性化剤とのARCの相互作用を決定する.
- 合成遺伝子の活性化を促進するARCの役割を明らかにする.
主な方法:
- クロマチン組み立てDNAテンプレートを使用したインビトロ転写アッセイ.
- ARCと転写活性化剤のタンパク質-タンパク質相互作用の分析.
- ARC複合体のサブユニットの特徴と,既知のコファクターとの比較.
主要な成果:
- ARCは,SREBP-1a,VP16,p65.5を含む複数のアクティベーターと直接相互作用する.
- ARCは,これらのアクティベーターによってクロマチンのテンプレートに誘導される転写を著しく強化します.
- ARCコンプレックスは16以上のサブユニットで構成されており,一部のサブユニットは新しいもので,他のサブユニットはCRSP,NAT,Mediatorと共有されています.
- ARCは,核ホルモン受容体共因子DRIPとほぼ同じである.
結論:
- ARC/DRIPは,トランスクリプション刺激に不可欠な大型複合コアクティベーターです.
- このコアクティベーターファミリーは,遺伝子の発現を媒介する多様なアクティベーターによって標的にされます.
- ARC/DRIPは,真核生物の遺伝子活性化の複雑な調節に重要な役割を果たしています.
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