RNA編集による代替スプライシングの規制

S M Rueter¹, T R Dawson, R B Emeson

¹Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-6600, USA.

Nature

|May 20, 1999

まとめ

酵素ADAR2は,そのプレ-mRNAを編集することによって,自身の発現を調節する. このRNA編集プロセスは,代替スプライシングを制御し,メッセンジャーRNAの産生に影響を与えます.

科学分野:

分子生物学は分子生物学である.
遺伝学遺伝学とは
RNA 生物学 RNA 生物学

背景:

ADAR2 (RNA2に作用するアデノシンデアミナーゼ) は,哺乳類のRNA編集に不可欠です.
RNA編集は,メッセンジャーRNAでアデノシンをイノシンに変換する.
代替スプライシングは,単一の遺伝子から多様なmRNAトランスクリプトを生成します.

研究の目的:

自分の遺伝子発現を調節するADAR2の役割を調査する.
ネズミのADAR2 mRNAにおける代替スプライシングイベントを特定するために.
RNA編集と代替スプライシングの相互作用を探求する.

主な方法:

ADAR2ゲノムDNAの核酸配列分析.ADAR2ゲノムDNAの核酸配列分析.ADAR2ゲノムDNAの核酸配列分析.ADAR2ゲノムDNAの核酸配列分析.ADAR2ゲノムDNAの核酸配列分析.ADAR2ゲノムDNAの核酸配列分析.ADAR2ゲノムDNAの核酸配列分析.ADAR2ゲノムDNAの核酸配列分析.ADAR2ゲノムDNAの核酸配列分析.
ADAR2 mRNAにおける代替スプライシング変異の識別.
ADAR2前mRNA.RNAにおけるRNA編集イベントの分析

主要な成果:

ネズミのADAR2mRNAで2つの異なる代替スプライシングイベントが特定されました.
側近3'のスプライス受容体部位の使用は,47-ヌクレオチド挿入につながります.
ADAR2は,独自のプレ-mRNAを編集し,内在的なAAをAIに変換し,AGスプライスサイトを模倣します.

結論:

ADAR2によるRNA編集は,独自のプレ-mRNAの代替スプライシングを直接制御する.
このメカニズムにより,ADAR2は自身の発現レベルを調節することができます.
ADAR2は,RNA編集とスプライシングを含む新しい自己調節戦略を採用しています.

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