人間の血管の滑らかな筋肉における変異性エストロゲンとプロゲステロン受容体メッセージ
Y K Hodges1, J K Richer, K B Horwitz
1Department of Medicine, Division of Cardiology, University of Colorado Health Sciences Center, Denver, Colo. 80262, USA.
Circulation
|May 25, 1999
まとめ
変異性エストロゲンとプロゲステロン受容体は,人間の血管の滑らかな筋肉に存在します. これらの変異は,性ホルモンが様々な組織で血管にどのように異なる影響を及ぼすかを説明する可能性がある.
科学分野:
- エンドクリノロジー エンドクリノロジー
- 分子生物学は分子生物学である.
- 血管生物学 血管生物学
背景:
- エストロゲンは乳腺/子宮細胞の成長を促しますが,血管の滑らかな筋肉を保護します.
- ホルモンの作用の変動性は,組織特異の受容体発現に起因する可能性があります.
- この研究では,ヒトの血管の滑らかな筋肉におけるエストロゲン受容体 (ERs) とプロゲステロン受容体 (PRs) を調査した.
研究 の 目的:
- 人間の血管の滑らかな筋肉におけるERとPRの構造を分析する.
- これらの受容体の潜在的な変異形態を特定するために.
- 血管組織における性ホルモンの作用に対する受容体変異の影響を理解する.
主な方法:
- 人間の血管の滑らかな筋肉からRNAの分離.
- リバーストランスクリプタゼとポリメラーゼ連鎖反応を用いたER-alphaとPR機能ドメインの特徴化.
- PRの変数の機能的特徴.
主要な成果:
- 野生型のER-alphaとPRを特定し,5種類のER-alphaと2種類のPRのトランスクリプトも確認した.
- 変異トランスクリプトは,ホルモン結合ドメインの削除を特徴付けている (例えば,PRDelta4,PRDelta6,ERDelta4,ERDelta5,ERDelta6,ERDelta7,ERDelta6,7).
- PRDelta6は,ワイルド型受容体の支配的陰性阻害体として作用した;変異PRは閉経前でも閉経後の女性では見つかりませんでした.
結論:
- 変種ERとPRのトランスクリプトは,人間の血管の滑らかな筋肉で広く表現されています.
- 異質な受容体形態は,複雑な組織特異的な性ホルモン効果を媒介する可能性があります.
- 変異型ERとPRの存在は,血管の滑らかな筋肉におけるエストロゲンとプロゲスティンの生理学的影響を変化させる可能性があります.
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