浸透性シリコンの脱吸収イオン化質量スペクトロメトリー

J Wei¹, J M Buriak, G Siuzdak

¹Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA.

Nature

|June 3, 1999

まとめ

質量スペクトロメトリのための新しいマトリックスフリー脱吸収イオン化方法は,多孔性のシリコン表面を使用しています. このテクニックは,生物分子および小分子分析のための感受性を高め,断片化を軽減します.

科学分野:

アナリティカル・ケミストリー (Analytical Chemistry) とは
表面科学とは,地表科学である.
バイオテクノロジーバイオテクノロジー

背景:

質量スペクトロメトリー技術は90年以上にわたって大きく進化してきました.
1980年代に有機マトリックスが導入されたことで,エネルギー伝送は改善されたが,背景イオンと断片化は増加した.
既存の方法は,しばしば感度と分析物の断片化に苦戦する.

研究の目的:

質量スペクトロメトリーのためのマトリックスフリー・デソルプション・イオン化戦略の開発.
感度を高め,分析剤の断片化を最小限に抑えるため.
バイオ分子と小分子の分析を可能にし,性能を向上させる.

主な方法:

分析物質の捕獲とパルスレーザー脱吸収イオン化のために,多孔性のシリコン表面を使用します.
レーザー照射を用いて,閉じ込められた分析物質を蒸発させ,イオン化する.
イオン化を最適化するために,多孔性のシリコンを化学的および構造的に改造する.

主要な成果:

フェムトモールとアトモールレベルの分析剤の分析が成功しました.
マトリックス・アシスト・メソッドと比較して,最小限の分析分断を証明した.
マトリックスがないため,小分子分析の適性を示した.
シリコンマイクロ流体と良好な感度と互換性を達成しました.

結論:

開発されたマトリックスフリー多孔シリコン方法は,質量スペクトロメトリーに敏感で低断片化のアプローチを提供します.
この技術は,バイオ分子および小分子分析の両方にとって有利です.
マイクロフリウイド技術との互換性は,高度な分析装置への道を開く.

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