フィブロブラスト成長因子による内皮から哺乳類の肝臓発達の開始

J Jung¹, M Zheng, M Goldfarb

¹Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Box G-J363, Providence, RI 02912, USA.

Science (New York, N.Y.)

|June 18, 1999

まとめ

線維細胞成長因子 (FGF) は肝臓の発達に重要な役割を果たします. FGF1とFGF2は肝臓の遺伝子発現を開始し,FGF8は胚の発達における肝臓の成長を促進する.

科学分野:

発達生物学発達生物学について
分子生物学は分子生物学である.
オルガノゲネシスオルガノゲネシス

背景:

哺乳類の腸内皮から胚性肝臓誘導を誘導する分子信号は未確認のままである.
フィブロブラスト成長因子 (FGFs) を発現する心臓内皮は,前腸内皮から肝臓の発達を誘発することが知られている.

研究の目的:

胚性肝臓誘導に責任を負う特定のシグナル伝達分子を特定する.
ヘパトゲネシスと肝臓外成長の開始における異なるFGFの異なる役割を解明する.

主な方法:

孤立したマウスの胚前腸内皮を,特定のFGFs (FGF1,FGF2,FGF8) で治療する.
ヘパトゲネシスに関連する遺伝子発現プログラムの分析.
内皮におけるFGF受容体発現の調査.
FGFと特定の阻害剤を用いて,発達段階を研究する.

主要な成果:

FGF1とFGF2は,FGF8ではないが,肝臓の遺伝子発現プログラムを誘発する心筋メソデルマの代わりになる可能性がある.
ヘパトゲン反応は,FGF受容体1と4を発現する内皮に特異的であった.
FGF8は,肝臓内皮の形態遺伝的成長に寄与することが判明しました.

結論:

異なるFGF信号は,哺乳類の肝臓発達の異なる段階をオーケストラする.
FGF1/FGF2は早期の肝発症を誘発し,FGF8は後の形態遺伝的プロセスを誘発する.

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