腫瘍ネクロシス因子-アルファ変換酵素と腫瘍ネクロシス因子-アルファは,ヒトの拡張性心筋病症における
M Satoh1, M Nakamura, H Saitoh
1Second Department of Internal Medicine, Iwate Medical University School of Medicine, Iwate, Japan. staohm@iwate-med.ac.jp
Circulation
|June 29, 1999
まとめ
腫瘍ネクロシス因子アルファ変換酵素 (TACE) 発現の増加は,拡張性心筋病 (DCM) の腫瘍ネクロシス因子アルファ (TNF-alpha) レベルの高さに関連しています. この関連性は,DCMのより重度の臨床指標と相関しています.
科学分野:
- 心臓病学 心臓病学
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
背景:
- 腫瘍死滅因子アルファ (TNF-alpha) は,拡張性心筋病 (DCM) の病原性に関与しています.
- TNF-α変換酵素 (TACE) は前駆体であるTNF-αを成熟した形に処理します.
- DCMにおける心筋TNF-α生成におけるTACEの役割は,完全に理解されていません.
研究 の 目的:
- DCM患者の心筋内におけるTACEおよびTNF-α発現を調査する.
- TACEとTNF-αのレベルとDCMの臨床的重症度の間の関係を決定する.
主な方法:
- 30人のDCM患者と5人の対照群からの心内組織サンプル.
- mRNAレベルに対するリアルタイムの定量的逆転写酵素-ポリメラーゼ連鎖反応.
- タンパク質発現のための免疫ヒスト化学分析.
主要な成果:
- TNF-αとTACE mRNAの両方が,対照群と比較して,DCM患者で有意に上昇しました.
- TNF-αとTACE mRNAレベル (r=0.779) の間で強い正の相関が観察されました.
- TACEとTNF-α発現の増加は,左心室上部の終末シストリック直径と左心室下部のエジェクション分数と相関しています.
結論:
- 心筋TACE発現の増加は,DCMにおける心筋TNF-α発現の増加と関連しています.
- この関連性は,mRNAとタンパク質のレベルの両方で明らかです.
- 研究結果は,TACEが臨床的に進行したDCMの進行に役割を果たすことを示唆しています.
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