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What is Genetic Engineering?00:49

What is Genetic Engineering?

Overview
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Transgenic Plants02:50

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Generator Voltage Control01:21

Generator Voltage Control

Generator voltage control is crucial for maintaining the stable operation of synchronous generators and wind turbines. In older models, a DC generator driven by the rotor delivers DC power to the rotor's field winding, and the power is transferred through slip rings and brushes. In the latest models, static or brushless exciters are used. Static exciters rectify AC power from the generator terminals and then transfer the DC power directly to the rotor. Brushless exciters, on the other hand, use...
Global Regulatory Systems01:28

Global Regulatory Systems

Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...

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関連する実験動画

Updated: Jul 21, 2026

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
06:21

An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness

Published on: May 7, 2018

"緑の革命"の遺伝子は,変異したギブベレリンの応答調節器をコードする.

J Peng1, D E Richards, N M Hartley

  • 1John Innes Centre, Norwich Research Park, UK.

Nature
|July 27, 1999
PubMed
まとめ

緑の革命とは

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科学分野:

  • 植物遺伝学 植物遺伝学
  • 分子生物学は分子生物学である.
  • 農業科学 農業科学とは

背景:

  • 緑の革命は,新しい半矮小の品種を用いて,世界の小麦の収穫量を大幅に増加させた.
  • これらの半矮小麦は,植物ホルモンGibberellinに対する反応性が低下しています.
  • 低身長 (Rht) 遺伝子,特にRht-B1とRht-D1は,この矮小化特性を授与する.

研究 の 目的:

  • 農作物の矮小化におけるギベレリンの無感性の分子基礎を調査する.
  • アラビドプシスGAI.に関連する他の種におけるオートロゴス遺伝子を特定する.
  • 農作物の収穫量の改善のためにこれらの遺伝子を操作する可能性を調査する.

主な方法:

  • 比較ゲノミクスを用いて,オートロゴス遺伝子を特定する.
  • Rht-B1/Rht-D1とトウモロコシd8.8.の変異アレル解析
  • 変異GAIアレルを持つトランスジェニックの米植物を用いた機能分析.

主要な成果:

  • Rht-B1/Rht-D1とトウモロコシd8は,アラビドプシスGAI.のオーソログとして識別されています.
  • これらの遺伝子の変異性アレルは,保存されたN端のギブベレリンのシグナル伝達領域を変化させます.
  • 変異GAIを持つトランスジェニックライスは,ギベレリンの反応が低下し,矮小化を示した.

結論:

  • GAI遺伝子とそのオートロゲンは,ギベレリンのシグナル伝達と植物の高さに重要な役割を果たします.
  • GAIのオルトロジの保存ドメインの変異は,ギベレリン反応の減少と矮小化につながります.
  • GAIのオーソログを操作することは,様々な種で作物の収穫を増やすための潜在的な戦略を提供します.