Otx2発現の尾関節の限界は,静脈管の組織者を位置づけている
V Broccoli1, E Boncinelli, W Wurst
1GSF-Research Centre for Environment and Health, Institute of Mammalian Genetics, Neuherberg, Germany.
Nature
|September 18, 1999
まとめ
ホメオボックス遺伝子であるOtx2は,
科学分野:
- 発達生物学 発達生物学とは
- 神経科学は神経科学である.
- 遺伝学 遺伝学とは
背景:
- ホメオボックス遺伝子Otx2は前神経管の発達において重要な役割を果たし,その発現境界は中脳/後脳交差点を定義する.
- 以前の研究では,Otx2の無活性化が,その発現領域内の発達に不可欠であることが示されました.
研究 の 目的:
- Otx2発現の尾関節限界が,静脈組織器の位置づけに不可欠であるかどうかを調査する.
- Otx2が中脳と後脳に影響するかどうかを判断する.
主な方法:
- En1場所へのノックイン戦略を使用して,推定の前部後脳におけるOtx2の胎外発現.
- 成人トランスゲン脳の形態学的および組織学的分析.
- 主要な神経発達マーカー (Wnt1,エフリン-A5,Pax2,Fgf-8,Gbx2) の発現パターンをモニタリングする.
主要な成果:
- トランスジェニックの子孫は小脳梗塞を発症した.
- 前頭小脳毛虫の有意な減少と下部のコリキュルの補償的な拡大が観察されました.
- 中脳,静脈組織,後脳マーカーの発現における尾関節のシフトは,後脳領域の推定領域にある.
結論:
- Otx2発現の尾関節の限界は,静脈間組織器を配置するのに十分である.
- Otx2の発現は,中脳/後脳領域内の尾部中脳運命をコードするのに役立ちます.
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