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3.3 A解像度でThermus aquaticusのコアRNAポリメラーゼの結晶構造が示されています
G Zhang1, E A Campbell, L Minakhin
1The Rockefeller University, New York, New York 10021, USA.
Cell
|September 28, 1999
まとめ
Thermus aquaticusのコアRNAポリメラーゼの結晶構造は,中央のチャネルを持つカニの爪の形を示しています. これは,重要なマグネシウムイオンと,RNA合成と酵素プロセシビティに不可欠な機能的部位を明らかにします.
科学分野:
- バイオケミストリー バイオケミストリー
- 構造生物学 構造生物学とは
- 分子生物学は分子生物学である.
背景:
- RNAポリメラーゼは,すべての生物における遺伝子転写に不可欠である.
- 核RNAポリメラーゼの構造を理解することは,その触媒機構の解読の鍵です.
- Thermus aquaticusは,バクテリア性および真核性RNAポリメラーゼを研究するためのモデルシステムを提供します.
研究 の 目的:
- Thermus aquaticusコアRNAポリメラーゼのX線結晶構造を決定するために.
- 触媒活性部位の位置と保存された機能的モチーフを特定するために.
- 転写プロセシビティと基板配送に関与する構造的特徴を解明する.
主な方法:
- 高解像度構造を取得するために,X線結晶学を用いた.
- 変異とクロスリンクの研究を通じて特定された保存されたモチーフと機能的な部位の分析.
- 転写における既知の機能的役割と構造的特徴の比較.
主要な成果:
- 構造は"カニの爪"の形を呈し,内部チャネルは27ミリメートル幅である.
- 触媒的に不可欠なMg2+イオンは,保存モチーフによってケラートされ,チャネルの背面壁に位置しています.
- トランスクリプションバブル,DNA/RNA結合,および基板配送を維持するための重要な機能部位は,チャネル内に位置しています.
結論:
- 決定された構造は,コアRNAポリメラーゼ機能の詳細な分子モデルを提供します.
- 保存されたMg2+結合モチーフは,多様な生命体における触媒活性に不可欠である.
- 構造的な洞察は,RNAポリメラーゼがどのようにプロセシビティを維持し,RNAを効率的に合成するかを説明します.
関連する概念動画
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Different Types of RNA Have the Same Basic Structure
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The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
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In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
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Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
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There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Bacterial RNA Polymerase
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...

