関連する実験動画
Updated: Jul 10, 2026

06:17
Engineered 3D Silk-collagen-based Model of Polarized Neural Tissue
Published on: October 23, 2015
プレキシン-ニューロピリン-1複合体は,機能的なセマフォリン-3A受容体を形成する
T Takahashi1, A Fournier, F Nakamura
1Department of Neurology, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
Cell
|October 16, 1999
まとめ
セマフォリン-3A (Sema3A) が軸索に結合する際には,神経ピリン-1 (NP-1) とプレキシン-1 (Plex-1) の複合体が関与する. このNP-1/Plex 1複合体は,NP-1単独ではなく,Sema3Aを媒介する.
科学分野:
- 神経科学は神経科学である.
- 細胞生物学 細胞生物学
背景:
- クラス1およびクラス3のセマフォリンは,反発を媒介する軸索誘導信号である.
- これらのセマフォリンは,異なる細胞表面タンパク質に結合し,複雑な受容体機構を示唆します.
研究 の 目的:
- セマフォリン-3A (Sema3A) シグナリングを媒介するプレキシン-1 (Plex 1) とニューロピリン-1 (NP-1) の相互作用を調査する.
- セマ3A誘発細胞応答におけるNP-1/Plex 1複合体の機能的役割を決定する.
主な方法:
- タンパク質複合体の形成を評価するための共免疫プレシピテーション.
- セマフォリン親和性を測定するためのリガンド結合測定法.
- 付着細胞を用いた細胞形態学アッセイ.
- 感覚神経細胞における支配的否定的アプローチ.
- タンパク質の再分配を視覚化するためのコンフォカル顕微鏡.
主要な成果:
- ニューロピリン-1 (NP-1) とプレキシン-1 (Plex 1) は安定した複合体を形成する.
- NP-1/Plex 1複合体は,NP-1単独よりもセマフォリン-3A (Sema3A) に対するより高い親和性を示しています.
- セマ3AがNP-1/Plex 1複合体と結合すると,NP-1のみと結合するところとは異なり,細胞の丸めが誘発されます.
- 支配的陰性Plex 1は,Sema3A誘発の成長の崩壊を抑制する.
- セマ3A治療により,NP-1とプレキシンが成長コンに集結する.
結論:
- 生理学的セマフォリン-3A (Sema3A) 受容体は,ニューロピリン-1 (NP-1) /プレキシン-1 (Plex 1) コンプレックスで構成されています.
- この複雑な形成は,Sema3A媒介の軸索誘導と細胞反応に不可欠です.
関連する概念動画
Cytoskeletal Linker Proteins - Plakins
Plakins are large proteins with binding domains for microtubules, microfilaments, intermediate filaments, and membrane-associated protein complexes at cell junctions. Plakin functions are evolutionarily conserved and are primarily involved in organizing the different components of the cytoskeleton by crosslinking them to each other and connecting them to the cell-matrix and cell adhesion complexes. They are also known to interact with signal transducers, serve as scaffolds for signaling...
Mechanism of Filopodia Formation
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Assembly of Signaling Complexes
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
IP3/DAG Signaling Pathway
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...
Intracellular Signaling Affects Focal Adhesions
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.

