CREB媒介の長期促進の一時的なニューロン全体の形態は,局所的なタンパク質合成によって特定のシナプスで安定させることができます

A Casadio¹, K C Martin, M Giustetto

¹Howard Hughes Medical Institute and Center for Neurobiology and Behavior College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.

Cell

|October 27, 1999

まとめ

セロトニン (5-HT) は,CREBと局所タンパク質合成を含む,アプリシアニューロンにおけるシナプス特異の長期的な促進を誘発する. 細胞全体の促進は一時的なもので,成長とは関係ありません.

科学分野:

神経科学は神経科学である.
細胞生物学細胞生物学
分子生物学は分子生物学である.

背景:

セロトニン (5-HT) は,学習と記憶に関与する重要な神経調節剤です.
シナプス可塑性は記憶形成の基礎ですが,長期的な変化の分子機構は複雑です.

研究の目的:

アプリシア感受性ニューロンにおけるセロトニン (5-HT) によって誘発されるシナプス特有の長期的な促進のメカニズムを調査する.
5-HTに対する反応としてシナプス固有の促進と細胞全体の促進を区別する.

主な方法:

Aplysiaの感覚神経と運動神経細胞の培養システムを利用しました.
特定のシナプスまたは細胞体にセロトニン (5-HT) を適用します.
CREB (cAMP応答要素結合タンパク質) とタンパク質合成の役割を調査した.

主要な成果:

1つのシナプスに繰り返し5-HTを投与すると,CREB媒介のシナプス特有の長期的な促進が誘発されます.
このシナプス固有の促進は,他のシナプスにも伝達される可能性があります.
細胞体での5-HTによって誘発された細胞全体の促進はCREB依存的であったが,一時的であり,成長と関連していなかった.
周辺シナプスでの単一の5HTパルスで,持続的な促進とシナプス固有の成長が誘発されました.

結論:

単一の5HTパルスは,長期的な促進のためにシナプスをマークし安定させる短期的なプロセスを開始します.
この安定化には,共性マークとラパミシンに敏感な局所的なタンパク質合成が含まれます.
シナプス固有の促進は,細胞全体の促進とは異なり,前者は持続的な成長と関連しています.

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