生化学ゲノミクスのアプローチで,遺伝子の産物の活性によって遺伝子を識別する
M R Martzen1, S M McCraith, S L Spinelli
1Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, NY 14642, USA.
Science (New York, N.Y.)
|November 5, 1999
まとめ
新しいゲノム戦略により,酵母遺伝子の機能を迅速に特定できる. この方法により,周期性フォスフォディエステラーゼとシトクロームcメチルトランスフェラーゼを含む3つの新しい生化学的活性が発見され,酵母遺伝学の理解が深まりました.
科学分野:
- 分子生物学は分子生物学である.
- イースト遺伝学 イースト遺伝学
- バイオケミストリー バイオケミストリー
背景:
- 遺伝子の機能を特定することは,細胞のプロセスを理解するために極めて重要です.
- 酵母遺伝子の機能識別のための既存の方法は,遅くて労働集約的である可能性があります.
研究 の 目的:
- 生化学活動をコードする酵母遺伝子を識別するための迅速で,敏感で,広く適用可能なゲノム戦略を開発する.
- イーストにおけるORFに関連した新しい生化学的機能を発見する.
主な方法:
- 6144種類の酵母菌株のライブラリが作成され,それぞれにユニークなオープンリーディングフレーム (ORF) があり,グルタチオンS-トランスファーゼ (GST) と融合しました.
- 菌株をプーリングし,GST-ORFを浄化し,プールは生化学的活動のために測定されました.
- アクティブプールは,その活動に責任を持つ特定のORFを特定するために分解されました.
主要な成果:
- 戦略は,これまで知られていなかったORF関連活動を成功裏に特定しました.
- 3つの新しい活性が発見されました:周期性アデノシン二酸化リボース酸塩 (Appr>p) に作用するサイクリック・フォスフォディエステラーゼ,Appr-1"-p処理活性,およびシトクロームcメチルトランスファーゼ.
結論:
- 開発されたゲノム戦略は,酵母遺伝子機能の高通量識別に有効です.
- このアプローチにより,特定の酵母ORFの新たな生化学的役割が発見され,酵母遺伝子の既知の機能的景観が拡大されました.
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