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Recombinant DNA

Recombinant DNA

PCR

PCR

Recombinant DNA

Recombinant DNA

PCR - Polymerase Chain Reaction

PCR - Polymerase Chain Reaction

RACE - Rapid Amplification of cDNA Ends

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

Plasmids

Plasmids

Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...

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Updated: Jul 8, 2026

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

遺伝子工学におけるベクトルレプリケータとしてのプラズミドRP4

A E Jacob, N J Grinter

|June 5, 1975

まとめ

No abstract available in PubMed .

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Last Updated: Jul 8, 2026

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

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Published on: October 18, 2022

A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector Construction

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Published on: May 17, 2024

CRISPR-based Shuttle Cloning: A High-throughput Cloning Method

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